Development of neuronal birthdate tagging

• Project/Area Number: 15K14323
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: National Institute of Genetics
• Principal Investigator: Hirata Tatsumi 国立遺伝学研究所, 総合遺伝研究系, 教授 (80260587)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 誕生日タグ / トランスジェニックマウス / 新規技術 / 神経発生 / 脳科学 / 誕生日タグづけ / マウス / 神経系 / 誕生日タグづけシステム / 神経回路

Outline of Final Research Achievements

Birth timing of neurons determines many characteristics in neurons and has been used for classification of neurons. This study developed transgenic mouse lines in which post-mitotic neurons can be genetically tagged in a birthdate-dependent manner. Four useful transgenic lines were selected and characterized in detail. Our results suggested that these lines can be used as powerful resource for neuronal classification and subsequent manipulation of the classified neuronal subset.

Research Products

• [Journal Article] The neocortical homologues in nonmammalian amniotes: bridging the hierarchical concepts of homology through comparative neurogenesis. (2017)
  • Author(s): Nomura T and Hirata T
  • Journal Title: Evolution of Nervous Systems Volume: 2 Pages: 195-204
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reallocation of olfactory Cajal-Retzius cells shapes neocortex architecture. (2016)
  • Author(s): de Frutos CA, Bouvier G, Arai Y, Thion MS, Lokmane L, Maryama K, Garcia-Dominguez M, Charnay P, Hirata T, Riethmacher D, Grove EA, Tissir F, Casado M, Pierani A, and Garel S
  • Journal Title: Neuron Volume: 92 Pages: 435-448
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Telomere Visualization in Tissue Sections Using Pyrrole-Imidazole Polyamide Probes. (2016)
  • Author(s): Sasaki, A., Ide, S., Kawamoto, Y., Bando, T., Murata, Y., Shimura, M., Yamada, K., Hirata, A., Nokihara, K., Hirata, T., Sugiyama, H., Maeshima, K.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 29261-29261
  • DOI: 10.1038/srep29261
  • NAID: 120005774690
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The COUP-TFII/Neuropilin-2 is a molecular switch steering diencephalon-derived GABAergic neurons in the developing mouse brain (2015)
  • Author(s): Shigeaki Kanatani, Takao Honda, Michihiko Aramaki, Kanehiro Hayashi, Ken-ichiro Kubo, Mami Ishida, Daisuke H. Tanaka, Takeshi Kawauchi, Katsutoshi Sekine, Sayaka Kusuzawa, Takahiko Kawasaki, Tatsumi Hirata, Hidenori Tabata, Per Uhlen and Kazunori Nakajima
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 112(36) Issue: 36
  • DOI: 10.1073/pnas.1420701112
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Transcallosal projections require glycoprotein M6-dependent neurite growth and guidance. (2014)
  • Author(s): S. Mita, P. Monasterio-Schrader, U. Fünfschilling, T. Kawasaki, H. Mizuno, T. Iwasato, K.-A. Nave, H. B. Werner, and T. Hirata
  • Journal Title: Cerebral Cortex Volume: [Epub ahead of print] Issue: 11 Pages: 4111-4125
  • DOI: 10.1093/cercor/bhu129
  • Peer Reviewed
• [Presentation] Evolutionary conservation of neocortical neurogenetic program in the mammals and birds (2015)
  • Author(s): T. Hirata
  • Organizer: 9th World Congress IBRO 2015, Symposium: Evolution of cerebral cortical development
  • Place of Presentation: Rio de Janeiro, Brazil
  • Year and Date: 2015-07-07
  • Int'l Joint Research / Invited
• [Presentation] Origin of the neurogenetic program in the mammalian neocortex (2015)
  • Author(s): T. Hirata
  • Organizer: 第48回日本発生生物学会 シンポジウム
  • Place of Presentation: 筑波
  • Year and Date: 2015-06-02
  • Invited