| Project/Area Number |
15K14336
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Imai Takeshi 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (70509851)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 蛍光イメージング / 神経回路 / コネクトーム / 透明化 |
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| Outline of Final Research Achievements |
To understand functional dynamics of the brain, it is important to analyze neuronal connectivity function in the same animals. In this study, we developed a method to stain thick brain tissues with antibodies and examine connectivity after tissue clearing. We found that high concentration of saponin solution facilitates antibody staining deep inside. We also developed a new tissue clearing agent that minimized spherical aberrations. Using this strategy, we established high-resolution fluorescence imaging of thick tissues.
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