Are there any highly oncogenic Epstein-Barr virus strains?

• Project/Area Number: 15K14391
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Tohoku Medical and Pharmaceutical University (2016); Aichi Cancer Center Research Institute (2015)
• Principal Investigator: KANDA Teru 東北医科薬科大学, 医学部, 教授 (50333472)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: EBウイルス / 胃がん / ゲノム編集 / クローン化 / 上皮細胞 / 発がん / EBV / 感染 / リンパ芽球様細胞株 / 大腸菌人工染色体

Outline of Final Research Achievements

Approximately 10% of gastric cancer cells are positive for Epstein-Barr virus (EBV) infection. Genome editing technology was used to clone full length genomes of two EBV strains derived from gastric cancer cell lines, and their sequences were determined. Phylogenetic analyses revealed that both strains belong to EBV family commonly observed in East Asian countries. When immortalized keratinocytes were stably infected with these gastric cancer-derived EBVs, the infected cells became resistant to oncogene-induced cell death, implicating the role of EBV infection in the initial phases of epithelial carcinogenesis.

Research Products

• [Journal Article] Elimination of LMP1-expressing cells from a monolayer of gastric cancer AGS cells. (2017)
  • Author(s): Sato Y, Ochiai S, Murata T, Kanda T, Goshima F, Kimura H.
  • Journal Title: Oncotarget Volume: 印刷中 Issue: 24 Pages: 39345-39355
  • DOI: 10.18632/oncotarget.16996
  • Peer Reviewed / Open Access
• [Journal Article] Highly efficient CRISPR/Cas9-mediated cloning and functional characterization of gastric cancer-derived Epstein-Barr virus strains (2016)
  • Author(s): Teru Kanda, Yuki Furuse, Hitoshi Oshitani, Tohru Kiyono
  • Journal Title: Journal of Virology Volume: 90 Issue: 9 Pages: 4383-4393
  • DOI: 10.1128/jvi.00060-16
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] CRISPR/Cas9-mediated cloning of entire EBV genome DNAs derived from latently-infected cells (2016)
  • Author(s): Kanda T, Furuse Y, Oshitani H, Kiyono T.
  • Organizer: 第64回 日本ウイルス学会学術集会
  • Place of Presentation: 札幌コンベンションセンター(北海道・札幌市）
  • Year and Date: 2016-10-24
• [Presentation] Cloning of gastric cancer-derived Epstein-Barr virus genomes by means of a genome editing technology (2016)
  • Author(s): Teru Kanda
  • Organizer: 第75回 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-10-07
• [Presentation] ゲノム編集技術を応用した胃がん由来 EB ウイルス株の単離と解析 (2016)
  • Author(s): 神田輝、清野透
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2016-10-06
• [Presentation] Development of highly efficient and widely applicable CRISPR/Cas9-mediated cloning method of EBV genomes and its application to EBV-positive gastric cancer cell lines (2016)
  • Author(s): Kanda T, Furuse Y, Oshitani H, Kiyono T.
  • Organizer: 17th International Symposium on Epstein Barr virus and associated diseases
  • Place of Presentation: チューリッヒ（スイス）
  • Year and Date: 2016-08-08
  • Int'l Joint Research
• [Presentation] Development of highly efficient and widely applicable CRISPR/Cas9-mediated cloning method of EBV genomes and its application to EBV-positive gastric cancer cell lines (2016)
  • Author(s): Teru Kanda, Yuki Furuse, Hitoshi Oshitani, Tohru Kiyono
  • Organizer: EBV 2016
  • Place of Presentation: Zurich, Switzerland
  • Year and Date: 2016-08-08
  • Int'l Joint Research
• [Presentation] ゲノム編集技術による胃がん由来EBウイルス株の単離と解析 (2016)
  • Author(s): 神田 輝
  • Organizer: 第30回 ヘルペスウイルス研究会
  • Place of Presentation: セミナーハウス クロス・ウェーブ府中（東京都・府中市）
  • Year and Date: 2016-06-18
• [Book] Human herpesviruses (2017)
  • Author(s): Teru Kanda
  • Publisher: Springer
• [Remarks] 東北医科薬科大学・医学部・微生物学
  • URL: http://www.tohoku-mpu.ac.jp/medicine/about/microbiology/
• [Patent(Industrial Property Rights)] 巨大環状ウイルスゲノムDNAの単離方法 (2016)
  • Inventor(s): 神田 輝
  • Industrial Property Rights Holder: 神田 輝
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-017387
  • Filing Date: 2016-02-01