• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of B cell receptors expressed on tumor infiltrating B cells by next generation sequencing

Research Project

Project/Area Number 15K14410
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Tumor therapeutics
Research InstitutionJuntendo University

Principal Investigator

TAKEDA KAZUYOSHI  順天堂大学, 医学(系)研究科(研究院), 准教授 (80272821)

Co-Investigator(Renkei-kenkyūsha) SUZUKI Ryuji  独立行政法人国立病院機構(相模原病院臨床研究センター), 診断・治療研究室, 室長 (70373470)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsB細胞レセプター / 抗体 / T細胞レセプター / 抗腫瘍抗体 / 腫瘍免疫 / 次世代型シークエンサー / 次世代シークエンサー
Outline of Final Research Achievements

We have examined B cell receptor (BCR)(antibody) expressed on tumor infiltrating B cells (BCRs) by next generation sequencing (NGS).In the tumors that were effectively rejected by immune therapies, BCR clonality was less. Besides, BCRs were highly oligoclonal when we examined TIBs in the immune therapy-resistant tumors, suggesting specific infiltration of TIBs. We now analyze whether alteration of BCR clonality is causes or results of the difference of therapeutic effects. Moreover, we made antibodies produced by TIBs that clonaly infiltrating in resistant tumors and antibodies produced by TIBs commonly observed in several rejected tumors, and we are now going to define their antigens.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2017 2016 2015 Other

All Int'l Joint Research (5 results) Journal Article (13 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 13 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Int'l Joint Research: 3 results)

  • [Int'l Joint Research] Garvan Institute of Medical Research/QIMR Berghofer Medical Research Inst./University of Queensland(Australia)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Icahn School of Medicine at Mount Sinai(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] QIMR Berghofer/Medical Research Institute(Australia)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] School of Medicine/University of Queensland(Australia)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] Washington University, St. Louis, MO/St. Louis, MO(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Effective induction of cytotoxic T cells recognizing an epitope peptide derived from hypoxia-inducible protein 2 (HIG2) in patients with metastatic renal cell carcinoma.2017

    • Author(s)
      Obara, W., Karashima, T., Takeda, K., Kato, R., Kato, Y., Kanehira, M., Takata, R., Inoue, K., Katagiri, T., Shuin, T., Nakamura, Y. & Fujioka, T.
    • Journal Title

      Cancer Immunol. Immunother.

      Volume: 66 Issue: 1 Pages: 17-24

    • DOI

      10.1007/s00262-016-1915-5

    • NAID

      120006894604

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IFN-γ is required for cytotoxic T cell-dependent cancer genome immunoediting.2017

    • Author(s)
      Takeda K, Nakayama M, Hayakawa Y, Kojima Y, Ikeda H, Imai N, Ogasawara K, Okumura K, Thomas DM, Smyth MJ
    • Journal Title

      Nat Commun.

      Volume: 8 Issue: 1 Pages: 14607-14607

    • DOI

      10.1038/ncomms14607

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Targeting cytokine signaling checkpoint CIS activates NK cells to protect from tumor initiation and metastasis.2017

    • Author(s)
      Putz, E. M., Guillerey, C., Kos, K., Stannard, K., Miles, K., Delconte, R. B., Takeda, K., Nicholson, S. E., Huntington, N. D. 6 Smyth, M. J.
    • Journal Title

      Oncoimmunology

      Volume: 6 Issue: 2 Pages: e1267892-e1267892

    • DOI

      10.1080/2162402x.2016.1267892

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Suppression of metastasis using a new lymphocyte checkpoint target for cancer immunotherapy.2016

    • Author(s)
      Blake, S. J., Stannard, K., Liu, J., Allen, S., Yong, M. C., Mittal, D., Aguilera, A. R., Miles, J. J., Lutzky, V. P., de Andrade, L. F., Martinet, L., Colonna, M., Takeda, K., Kuhnel, F., Gurlevik, E., Bernhardt, G., Teng, M. W., & Smyth, M. J.
    • Journal Title

      Cancer Discov.

      Volume: 6 Issue: 4 Pages: 446-459

    • DOI

      10.1158/2159-8290.cd-15-0944

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Phase I clinical trial of a five-peptide vaccine combined with cyclophosphamide in advanced solid tumors.2016

    • Author(s)
      Murahashi, M., Hijikata, Y., Yamada, K., Tanaka, Y., Kishimoto, J., Inoue, H., Marumoto, T., Takahazhi, A., Okazaki, T., Takeda, K., Hirakawa, M., Fujii, H., Okano, S., Morita, M., Baba, E., Mizumoto, K., Maehara, Y., Tanaka, M., Akashi, K., Nakanishi, Y., Yoshida, K., Tsunoda, T., Tamura, K., Nakamura, Y., & Tani, K.
    • Journal Title

      Clin. Immunol.

      Volume: 166-167 Pages: 48-58

    • DOI

      10.1016/j.clim.2016.03.015

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Co-inhibition of CD73 and A2AR adenosine signaling improves anti-tumor immune responses.2016

    • Author(s)
      Young, A., Ngiow, S. F., Barkauskas, D. S., Sult, E., Hay, C., Stephen J. Blake, S. J., Huang, Q., Liu, J., Takeda, K., Teng, M. W. L., Sachsenmeier, K., & Smyth, M. J.
    • Journal Title

      Cancer Cell

      Volume: 30 Issue: 3 Pages: 391-403

    • DOI

      10.1016/j.ccell.2016.06.025

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Assessing immune-related adverse events of efficacious combination immunotherapies in preclinical models of cancer.2016

    • Author(s)
      Liu, J., Blake, S. J., Harjunpaa, H., Fairfax, K. A., Yong, M. C., Allen, S., Kohrt, H. E., Takeda, K., Smyth, M. J., & Teng, M. W. L.
    • Journal Title

      Cancer Res.

      Volume: 76 Issue: 18 Pages: 5288-5301

    • DOI

      10.1158/0008-5472.can-16-0194

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Improved efficacy of neoadjuvant compared to adjuvant immunotherapy to eradicate metastatic disease.2016

    • Author(s)
      Liu, J., Blake, S. J., Yong, M. C., Harjunpaa, H., Ngiow, S. F., Takeda, K., Young, A., O’Donell, J. S., Allen, S., Smyth, M.J., & Teng, M. W. L.
    • Journal Title

      Cancer Discov.

      Volume: 6 Issue: 12 Pages: 1382-1399

    • DOI

      10.1158/2159-8290.cd-16-0577

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Agonists of the TRAIL death receptor DR5 sensitize intestinal stem cells to chemotherapy-induced cell death and trigger gastrointestinal toxicity.2016

    • Author(s)
      Finnberg, N. K., Gokare, P., Navaraj, A., Lang Luhs K. A., Cerniglia, G., Yagita, H., Takeda, K., Motoyama, N., and EL-Deiry, W. S.
    • Journal Title

      Cancer Res.

      Volume: 76 Issue: 3 Pages: 700-712

    • DOI

      10.1158/0008-5472.can-15-2759

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] NK cells require IL-28R for optimal in vivo activity.2015

    • Author(s)
      Souza-Fonseca-Guimaraes, F., Young, A., Mittal, D., Martinet, L., Bruedigam, C., Takeda, K., Degli-Esposti, M., Andoniou, C. E., Hill, G. R. and Smyth, M.J.
    • Journal Title

      Proc. Natl. Acad. Sci. USA.

      Volume: 112 Issue: 18

    • DOI

      10.1073/pnas.1424241112

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] High-dose cyclophosphamide induces specific tumor immunity with concomitant recruitment of LAMP1/CD107a-expressing CD4-positive T cells into tumor sites.2015

    • Author(s)
      Naito, T., Baba, T., Takeda, K., Sasaki, S., Nakamoto, Y. and Mukaida, N.
    • Journal Title

      Cancer Letters

      Volume: 366 Issue: 1 Pages: 93-99

    • DOI

      10.1016/j.canlet.2015.06.009

    • NAID

      120005663501

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Toll-Like Receptor 3 regulates NK cell responses to cytokines and controls experimental metastasis.2015

    • Author(s)
      Guillerey, C., Chow, M. T., Miles, K., Olver, S., Sceneay, J., Takeda, K., Mollere, A. and Smyth, M.J.
    • Journal Title

      OncoImmunology

      Volume: 4 Issue: 9 Pages: e1027468-e1027468

    • DOI

      10.1080/2162402x.2015.1027468

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Interferon-γ-Mediated Natural Killer Cell Activation by an Aqueous Panax ginseng Extract.2015

    • Author(s)
      Takeda, K. and Okumura, K.
    • Journal Title

      eCAM

      Volume: 2015 Pages: 1-11

    • DOI

      10.1155/2015/603198

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Most of the CD8+ TIL are tumor reactive in spontaneously regressing or progressing murine gastric cancer2015

    • Author(s)
      Nagaoka, K., Hosoi, A., Matsushita, H., Kubo, M., Takeda, K., Kakimi, K.
    • Organizer
      第44回日本免疫学会学術総会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Carcinoma-associated fibroblasts raise highly aggressive cancer cells via metastasis-promoting autocrine signaling2015

    • Author(s)
      Matsumura, Y., Ito, Y., Wali, N., Ito, T, Mogushi, K., Terao, Y., Takeda, S., Okumura, K., Takeda, K., Hino, O., Orimo, A.
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2015-10-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] IFN-γ produced by cytotoxic T cells immunoedits the cancer genome2015

    • Author(s)
      Takeda, K.
    • Organizer
      International conference of cancer immunotherapy and macrophage 2015
    • Place of Presentation
      東京都文京区
    • Year and Date
      2015-07-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2015-04-16   Modified: 2022-02-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi