The establishment of cell maturation induction method by the gene body-centered epigenomic monitoring.
Project/Area Number |
15K14431
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Medical genome science
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Research Institution | Keio University |
Principal Investigator |
Oda Mayumi 慶應義塾大学, 医学部(信濃町), 助教 (80567511)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | DNAメチル化 / 酸化的脱メチル化過程 / gene body領域 / 転写伸長反応 / 細胞成熟 / 心筋細胞 / ゲノム / エピゲノム / H3K36トリメチル化 / Gene body領域 / ゲノムワイド / 細胞分化 / 幹細胞 / 転写伸長 |
Outline of Final Research Achievements |
This research project aims to evaluate and induce cell maturation on the basis of the possibility that the epigenetic state of the gene body region may be involved in cell maturation in a mammalian genome whose background is global DNA methylation modification. Comparison of cardiomyocytes and hepatocytes prior to the establishment of the initial maturation induction method revealed that the gene body epigenetic domain characterized by DNA hypomethylation is evident in cardiomyocytes and less pronounced in other cell types. Analysis of DNA demethylation metabolic intermediates in this cardiomyocyte-specific epigenetic domain also showed that the oxidative demethylation process might be involved in the construction of this active epigenetic domains.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Violet Light Exposure Can Be a Preventive Strategy Against Myopia Progression.2017
Author(s)
Torii H, Kurihara T, Seko Y, Negishi K, Ohnuma K, Inaba T, Kawashima M, Jiang X, Kondo S, Miyauchi M, Miwa Y, Katada Y, Mori K, Kato K, Tsubota K, Goto H, Oda M, Hatori M, Tsubota K.
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Journal Title
EBioMedicine
Volume: 15
Pages: 210-219
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Epigenetic manipulation facilitates the generation of skeletal muscle cells from pluripotent stem cells.2017
Author(s)
Tomohiko Akiyama, Shunichi Wakabayashi, Atsumi Soma, Saeko Sato, Yuhki Nakatake, Mayumi Oda, Miyako Murakami, Miki Sakota, Nana Chikazawa-Nohtomi, Shigeru B. H. Ko, Minoru S. H. Ko
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Journal Title
Stem Cell International.
Volume: 2017
Pages: 7215010-7215010
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Salt suppresses IFNγ inducible chemokines through the IFNγ-JAK1-STAT1 signaling pathway in proximal tubular cells.2017
Author(s)
Arai Y, Takahashi D, Asano K, Tanaka M, Oda M, Ko SBH, Ko MSH, Mandai S, Nomura N, Rai T, Uchida S, Sohara E.
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Journal Title
Sci Rep.
Volume: 7
Issue: 1
Pages: 46580-46580
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Transient ectopic expression of the histone demethylase JMJD3 accelerates the differentiation of human pluripotent stem cells.2016
Author(s)
Tomohiko Akiyama, Shunichi Wakabayashi, Atsumi Soma, Saeko Sato, Yuhki Nakatake, Mayumi Oda, Miyako Murakami, Miki Sakota, Nana Chikazawa-Nohtomi, Shigeru B. H. Ko, Minoru S. H. Ko.
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Journal Title
Development
Volume: 143
Issue: 20
Pages: 3674-3685
DOI
Related Report
Peer Reviewed
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[Journal Article] H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells2016
Author(s)
Kunitomi A, Yuasa S, Sugiyama F, Saito Y, Seki T, Kusumoto D, Kashimura S, Takei M, Tohyama S, Hashimoto H, Egashira T, Tanimoto Y, Mizuno S, Tanaka S, Okuno H, Yamazawa K, Watanabe H, Oda M, Kaneda R, Matsuzaki Y, Nagai T, Okano H, Yagami K, Tanaka M, Fukuda K.
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Journal Title
Stem Cell Reports
Volume: 6
Issue: 6
Pages: 825-833
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] H1foo has a pivotal role in qualifying induced pluripotent stem cells2016
Author(s)
Akira Kunitomi, Shinsuke Yuasa, Fumihiro Sugiyama, Yuki Saito, T Seki, D Kusumoto, S Kashimura, M Takei, S Tohyama, H Hashimoto, T Egashira, Yoko Tanimoto, Saori Mizuno, S Tanaka, H Okuno, K Yamazawa, H Watanabe, M Oda, R Kaneda, Y Matsuzaki, H Okano, Ken-ichi Yagami, M Tanaka, K Fukuda
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Journal Title
Stem Cell Research
Volume: 印刷中
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells.2015
Author(s)
Akiyama T, Xin L, Oda M, Sharov AA, Amano M, Piao Y, Cadet JS, Dudekula DB, Qian Y, Wang W, Ko SB, Ko MS
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Journal Title
DNA research (Oxford Journals)
Volume: 22
Issue: 5
Pages: 307-318
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Gene-body epigenetic domains participate in the coordination of gene-length heterogeneity in cardiomyocyte-specific gene transcription.2017
Author(s)
Mayumi Oda, Shunichi Wakabayashi, N. Ari Wijetunga, Shinsuke Yuasa, Hirokazu Enomoto, Ruri Kaneda, Sung Han Yoon, Nishant Mittal, Qiang Jing, Masako Suzuki, John M. Greally, Keiichi Fukuda and Shinji Makino.
Organizer
Keystone Symposia: Heart development
Place of Presentation
Keysone, CO, USA
Year and Date
2017-03-26
Related Report
Int'l Joint Research
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