2016 Fiscal Year Final Research Report
The establishment of cell maturation induction method by the gene body-centered epigenomic monitoring.
Project/Area Number |
15K14431
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Medical genome science
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Research Institution | Keio University |
Principal Investigator |
Oda Mayumi 慶應義塾大学, 医学部(信濃町), 助教 (80567511)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Keywords | DNAメチル化 / 酸化的脱メチル化過程 / gene body領域 / 転写伸長反応 / 細胞成熟 / 心筋細胞 / ゲノム / エピゲノム |
Outline of Final Research Achievements |
This research project aims to evaluate and induce cell maturation on the basis of the possibility that the epigenetic state of the gene body region may be involved in cell maturation in a mammalian genome whose background is global DNA methylation modification. Comparison of cardiomyocytes and hepatocytes prior to the establishment of the initial maturation induction method revealed that the gene body epigenetic domain characterized by DNA hypomethylation is evident in cardiomyocytes and less pronounced in other cell types. Analysis of DNA demethylation metabolic intermediates in this cardiomyocyte-specific epigenetic domain also showed that the oxidative demethylation process might be involved in the construction of this active epigenetic domains.
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Free Research Field |
エピゲノム学
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