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Experimental verification of hidden amyloidogenicity of proteins

Research Project

Project/Area Number 15K14458
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

Goto Yuji  大阪大学, たんぱく質研究所, 教授 (40153770)

Co-Investigator(Renkei-kenkyūsha) OGI Hirotsugu  大阪大学, 基礎工学研究科, 准教授 (90252626)
SAKURAI Kazumasa  近畿大学, 先端技術総合研究所, 准教授 (10403015)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords蛋白質 / 脳神経変性疾患 / 生体分子 / 凝集 / 変性 / アミロイド線維 / 溶解度 / 過飽和 / 脳神経疾患
Outline of Final Research Achievements

Various proteins form amyloid fibrils and are responsible for amyloidosis. On the other hand, other proteins do not form amyloid fibrils. If we could form amyloid fibrils with those non-amyloid proteins, we can advance our understanding of the mechanism of amyloid formation.
Ovalbumin consisting of 385 amino acid residues is one of the most popular food proteins and does not form amyloid fibrils. We found that a peptide mixture of ovalbumin produced by tryptic digestion contains an amyloidogenic peptide. We purified and determine the amino acid sequence of the amyloidogenic peptide. We also chemically synthesized the peptide and characterized its amyloidogenic properties. The results showed that even ovalbumin contains highly amyloidogenic sequence, although its amyloidogenicity is hidden by the suppressing effects of surrounding sequences.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (17 results)

All 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (9 results) (of which Int'l Joint Research: 6 results,  Invited: 9 results) Remarks (2 results)

  • [Int'l Joint Research] Eotvas Lorand University(ハンガリー)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] Australian National University(オーストラリア)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] Technische Universitat Munchen(ドイツ)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Heparin-induced amyloid fibrillation of beta2-microglobulin explained by solubility and a supersaturation-dependent conformational phase diagram.2017

    • Author(s)
      So, M., Hata, Y., Naiki, H. and Goto, Y.
    • Journal Title

      Protein Science

      Volume: 26 Issue: 5 Pages: 1024-1036

    • DOI

      10.1002/pro.3149

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Revisiting supersaturation as a factor determining amyloid fibrillation.2016

    • Author(s)
      So, M., Hall, D. and Goto, Y.
    • Journal Title

      Curr. Opin. Struct. Biol.

      Volume: 36 Pages: 32-39

    • DOI

      10.1016/j.sbi.2015.11.009

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Supersaturation-limited and unlimited phase transitions compete to produce the pathway complexity in amyloid fibrillation.2015

    • Author(s)
      Adachi, M. So, M., Sakurai, K., Kardos, J. and Goto, Y.
    • Journal Title

      J. Biol. Chem.

      Volume: 290 (29) Issue: 29 Pages: 18134-18145

    • DOI

      10.1074/jbc.m115.648139

    • NAID

      120006557633

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 超音波に依存した蛋白質過飽和溶液の相転移現象2017

    • Author(s)
      後藤祐児
    • Organizer
      日本物理学会第72回年次大会シンポジウム
    • Place of Presentation
      大阪大学(大阪府、豊中市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Revisiting supersaturation as a factor determining amyloid fibrillation2016

    • Author(s)
      Goto, Yuji
    • Organizer
      Third International Symposium on Protein Folding & Dynamics
    • Place of Presentation
      NCBS (Bangalore, India)
    • Year and Date
      2016-11-08
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] アモルファス凝集とアミロイド線維の区別および疾患関連性2016

    • Author(s)
      後藤祐児
    • Organizer
      企業現場のニーズと先端蛋白質科学との接点を探るシンポジウム
    • Place of Presentation
      東京大学(東京都、文京区)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 蛋白質の凝集と病気2016

    • Author(s)
      後藤祐児
    • Organizer
      大阪大学リサーチクラウドカフェ
    • Place of Presentation
      京阪電車なにわ橋駅(大阪府、大阪市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Toward understanding the unified mechanism of protein aggregation.2015

    • Author(s)
      Yuji Goto
    • Organizer
      農芸化学会中部関西合同大会・シンポジウム:酵素活性分子研究の新展開-タンパク質のフォールディング研究と酵素機能の利用
    • Place of Presentation
      富山県立大学(富山県射水市)
    • Year and Date
      2015-09-19
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Supersaturation-limited protein misfolding revealed by ultrasonication.2015

    • Author(s)
      Yuji Goto
    • Organizer
      IUBMB-SBBq Satellite Symposium: Frontiers in Protein Misfolding in Neurodegenerative Diseases and Cancer
    • Place of Presentation
      Rio de Janeiro (Brazil)
    • Year and Date
      2015-08-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Supersaturation-limited protein misfolding revealed by ultrasonication.2015

    • Author(s)
      Yuji Goto
    • Organizer
      The 23rd International Union of Biochemistry and Molecular Biology (IUBMB) Congress
    • Place of Presentation
      Foz do Iguacu (Brazil)
    • Year and Date
      2015-08-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] アミロイド線維形成における過飽和の果たす役割2015

    • Author(s)
      後藤祐児
    • Organizer
      第3回日本アミロイドーシス研究会学術集会:シンポジウム「アミロイド形成機構の解明と新たな治療戦略への展開」
    • Place of Presentation
      KKRホテル東京(東京)
    • Year and Date
      2015-08-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Supersaturation-limited and unlimited phase transitions compete to produce the pathway complexity in amyloid fibrillation.2015

    • Author(s)
      Yuji Goto
    • Organizer
      SFB1035 Conference on Conformational Transitions in Proteins
    • Place of Presentation
      Venice (Italy)
    • Year and Date
      2015-05-03
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 大阪大学・蛋白質研究所・蛋白質構造形成研究室

    • URL

      http://www.protein.osaka-u.ac.jp/physical/yoeki.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 大阪大学蛋白質研究所、蛋白質構造形成研究室

    • URL

      http://www.protein.osaka-u.ac.jp/laboratories/yoeki/

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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