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Analysis of budding yeast E3 ubiquitin ligases that regulate the degradation of short life protein

Research Project

Project/Area Number 15K14474
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionNagoya University

Principal Investigator

Kamura Takumi  名古屋大学, 理学研究科, 教授 (40333455)

Co-Investigator(Kenkyū-buntansha) 中務 邦雄  名古屋大学, 理学研究科, 講師 (90547522)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsタンパク質分解
Outline of Final Research Achievements

In this study, we searched for E3s which control the stability of the substrates to clarify various phenomenon regulated by the ubiquitin proteasome system. We chose several functionally important short life proteins using a database for protein half-life of the budding yeast. We identified E3s which controlled the degradation of these proteisn using E3 deletion strains. As a result, we found p70, p75, p75, p65 and p150 and p340 as E3s which controlled the degradation of Nup1, Tma17, Hcm1, Cdc1 and Spo12. In addition, we clarified the physiological significance of the Spo12 degradation mediated by p340. Our findings shed the light on the new function of ubiquitin proteasome system.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (20 results)

All 2017 2016 2015 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (14 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation.2016

    • Author(s)
      Uematsu K, Okumura F, Tonogai S, Joo-Okumura A, Alemayehu DH, Nishikimi A, Fukui Y, Nakatsukasa K, Kamura T.
    • Journal Title

      Journal of Cell Biology

      Volume: 215 Issue: 1 Pages: 95-106

    • DOI

      10.1083/jcb.201603062

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Parallel Regulation of von Hippel-Lindau Disease by pVHL-Mediated Degradation of B-Myb and Hypoxia-Inducible Factor α.2016

    • Author(s)
      Okumura F, Uematsu K, Byrne SD, Hirano M, Joo-Okumura A, Nishikimi A, Shuin T, Fukui Y, Nakatsukasa K, Kamura T.
    • Journal Title

      Molecular Cellular Biology

      Volume: 36 Issue: 12 Pages: 1803-1817

    • DOI

      10.1128/mcb.00067-16

    • NAID

      120005844088

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Subcellular Fractionation Analysis of the Extraction of Ubiquitinated Polytopic Membrane Substrate during ER-Associated Degradation.2016

    • Author(s)
      Nakatsukasa, K., Kamura, T
    • Journal Title

      Plos one

      Volume: 11 Issue: 2 Pages: 1-17

    • DOI

      10.1371/journal.pone.0148327

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The ubiquitin ligase SCFUcc1 acts as a metabolic switch for the glyoxylate cycle.2015

    • Author(s)
      Nakatsukasa, K., Nishimura, T., Byrne, D., Okamoto, M., Takahashi-Nakaguchi, A., Chibana, H., Okumura, F., Kamura, T.
    • Journal Title

      Molecular Cell

      Volume: 59 Issue: 1 Pages: 22-34

    • DOI

      10.1016/j.molcel.2015.04.013

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Proteolytic regulation of metabolic enzymes by E3 ubiquitin ligase complexes: lessons from yeast.2015

    • Author(s)
      Nakatsukasa, K., Okumura, F., Kamura, T
    • Journal Title

      Crit Rev Biochem Mol Biol.

      Volume: 50 Issue: 6 Pages: 489-502

    • DOI

      10.3109/10409238.2015.1081869

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] pVHL依存的なB-MybとHIFαの分解はそれぞれ 独立してVHL病を制御している2017

    • Author(s)
      奥村 文彦, 植松 桂司, Byrne STUART D., 平野 みえ, 奥村(城尾) 晶子, 錦見 昭彦, 執印 太郎, 福 井 宣規, 中務 邦雄, 嘉村 巧
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      東北大学、仙台国際センター
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 出芽酵母E3リガーゼSCFDia2の新奇基質Prl1の同定2016

    • Author(s)
      Alemayehu Dawit Hailu、中務邦雄、奥村文彦、嘉村巧
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] ユビキチンリガーゼASB7によるDDA3の発現制御は適切な紡錘体形成と染色体分配に必要である2016

    • Author(s)
      奥村 文彦、植松 桂司、外海 駿輔、奥村(城尾) 晶子、Alemayehu Dawit Hailu、錦見 昭彦、福井 宣規、中務 邦雄、嘉村 巧
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 出芽酵母E3リガーゼ複合体SCFMet30によるAry34の分解制御2016

    • Author(s)
      山口竜、中務邦雄、奥村文彦、嘉村巧
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 出芽酵母タンパク質BDF2の機能解析2016

    • Author(s)
      大谷悠貴、中務邦雄、奥村文彦、嘉村巧
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 出芽酵母におけるトリアシルグリセロールリパーゼの活性制御機構の解析2016

    • Author(s)
      藤澤宗隆、中務邦雄、奥村文彦、嘉村巧
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] リボソーム生合成を負に調節する転写制御因子Dot6およびTod6は窒素源飢餓条件でプロテアソーム依存的に分解される2016

    • Author(s)
      鈴木優太、栗田英奈、中務邦雄、奥村文彦、嘉村巧
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] The Ubiquitin Ligase SCFUcc1 Acts as a Metabolic Switch for the Glyoxylate Cycle2016

    • Author(s)
      中務 邦雄、嘉村 巧
    • Organizer
      ICY14 (The 14th International Congress on Yeasts)
    • Place of Presentation
      淡路夢舞台国際会議場
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] Fボックスタンパク質Ucc1による代謝制御機構の解析2015

    • Author(s)
      中務 邦雄、嘉村 巧
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] pVHL依存的なFOBとHIFαの分解はそれぞれ独立してVHL病を制御している2015

    • Author(s)
      奥村 文彦、植松 桂司、Stuart D. Byrne、平野 みえ、奥村(城尾) 晶子、錦見 昭彦、執印 太郎、福井 宣規、中務 邦雄、嘉村 巧
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 染色体安定性の維持におけるCRL5ASB7ユビキチンリガーゼの機能2015

    • Author(s)
      植松 桂司、奥村 文彦、中務 邦雄、嘉村 巧
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] ユビキチンリガーゼSCFMet30の新規基質p17の同定と機能解析2015

    • Author(s)
      富澤 優貴、中務 邦雄、奥村 文彦、嘉村 巧
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 出芽酵母E3リガーゼSCFDia2の新奇基質Prl1の同定2015

    • Author(s)
      Dawit Hailu Alemayehu、中務 邦雄、奥村 文彦、嘉村 巧
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Hect型E3リガーゼTom1によるFEARネットワーク構成因子Spol2の分解制御2015

    • Author(s)
      栗田 英奈、中務 邦雄、曽根 恵、奥村 文彦、嘉村 巧
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Remarks] 分子修飾学教室へようこそ

    • URL

      http://bunshi4.bio.nagoya-u.ac.jp/~2kamura/index.html

    • Related Report
      2016 Annual Research Report 2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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