| Project/Area Number |
15K14478
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
TAJIMA Shoji 大阪大学, たんぱく質研究所, 教授 (50132931)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Hironobu 大阪大学, 蛋白質研究所, 助教 (60378891)
SUETAKE Isao 大阪大学, 蛋白質研究所, 准教授 (80304054)
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| Co-Investigator(Renkei-kenkyūsha) |
ITO Takashi 九州大学, 大学院医学研究院, 教授 (90201326)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | DNAヒドロキシメチル化修飾 / DNAメチル化 / DNAメチル基転移酵素 / DNAメチル化酵素 |
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| Outline of Final Research Achievements |
Genomic DNA methylation modification plays crucial role in development. Major players of the establishment of DNA methylation and replication-coupled maintenance DNA methylation have been identified. Recently, active DNA demethylation step has been reported to be catalyzed initially by TET family oxidases to produce hydroxymethylcytosine. Although several techniques to identify the position of hydroxymethylcytosine in genome have been reported, they possess disadvantages. In the present study, we have developed a new technique to determine hydroxymethylcytosine at single base resolution utilizing recombinant DNA methyltransferase DNMT1.
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