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Engineered Themosynechococcus elongatus mutant growing under photoheterotrophic conditions

Research Project

Project/Area Number 15K14492
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionEhime University

Principal Investigator

Sugiura Miwa  愛媛大学, プロテオサイエンスセンター, 准教授 (80312255)

Research Collaborator Rappaport Fabrice  フランス国立科学研究所, IBPC, 研究員
Boussac Alain  フランス国立科学研究所, CEA/Saclay, 主席研究員
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords光合成 / 光化学系II / 好熱性シアノバクテリア / 光従属生用成長 / 光従属栄養
Outline of Final Research Achievements

Energy conversion and water oxidation in photosynthesis are quite efficiently performed in plants and cyanobacteria. Understanding about these molecular mechanisms based on the molecular structure must be important subject not only from scientific point of view but also from the view point of new energy creation. Especially understanding relationship between molecular structure and function of Photosystem II, which plays initial reaction of photosynthetic electron transfer and water oxidation, is one of the most important subject. Although analyses of site-directed mutants are quite powerful approaches, we have not obtained important mutants of thermophilic cyanobacterial because of loss of photosynthetic function by mutations. For solution of these serious problems, in this study, we established new genetic engineering system of the thermophilic cyanobacterium, Thermosynechococcus elongatus.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 3 results) Presentation (3 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Book (1 results)

  • [Int'l Joint Research] CNRS(フランス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] CNRS(フランス)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Crystal structure and redox properties of a novel cyanobacterial heme-protein with a His/Cys heme axial ligation and a per-arnt-sim (PAS)-like domain2017

    • Author(s)
      Motomura, T., Suga, M., Hienerwadel, R., Nakagawa, A., Lai, T.-L., Nitschke, W., Sugiura, M, Boussac, A. and Shen, J.-R.
    • Journal Title

      J. Biol. Chem

      Volume: 印刷中

    • NAID

      120006473741

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Corrigendum to “Influence of Histidine-198 of the D1 subunit on the properties of the primary electron donor, P680, of Photosystem II in Thermosynechococcus elongatus”2016

    • Author(s)
      Sugiura, M., Ozaki, Y., Rappaport F., and Boussac A.
    • Journal Title

      Biochim. Biophys. Acta (Bioenergetics)

      Volume: 1857 Issue: 12 Pages: 1943-1948

    • DOI

      10.1016/j.bbabio.2016.09.012

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Assembly of oxygen-evolving Photosystem II efficiently occurs with the apo-Cytb559 alone but the holo-Cytb559 increases the recovery rate of a functional enzyme upon photo-inhibition.2015

    • Author(s)
      Sugiura, M., Nakamura, M., Koyama, K., and Boussac, A.
    • Journal Title

      Biochim. Biophys. Acta (Bioenergetics),

      Volume: 1847 Issue: 2 Pages: 276-285

    • DOI

      10.1016/j.bbabio.2014.11.009

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Molecular function and structure of Photosystem II in photosynthetic electron transfer2017

    • Author(s)
      Miwa Sugiura
    • Organizer
      Solar Fuel Material -Maltiscale and Bioinspired Approach in Catalyst-
    • Place of Presentation
      Seoul National University, Korea
    • Year and Date
      2017-02-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Role of D1-Pro173 of Photosystem II in Water Oxidation2016

    • Author(s)
      Miwa Sugiura
    • Organizer
      7th International Conference Photosynthesis Research for Sustainability-2016
    • Place of Presentation
      Pushchino, Russia
    • Year and Date
      2016-06-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Engineered Themosynechococcus elongatus mutant growing under photoheterotrophic conditions2015

    • Author(s)
      Miwa Sugiura
    • Organizer
      Gordon Research Conference: Photosynthesis
    • Place of Presentation
      Boston, U.S.A.
    • Year and Date
      2015-06-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 光合成のエネルギー変換と物質変換 ~人工光合成をめざして2015

    • Author(s)
      杉浦美羽
    • Total Pages
      281
    • Publisher
      化学同人
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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