| Project/Area Number |
15K14530
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
Nakamura Akira 熊本大学, 発生医学研究所, 教授 (90323245)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ショウジョウバエ / CRISPR-Cas9 / miRNA / ゲノム編集 / 母性-胚性遷移 / マイクロRNA |
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| Outline of Final Research Achievements |
The maternal-to-Zygotic transition (MZT) involves degradation of maternal product to switch zygotic gene-oriented development, and is the conserved process in animal development. To investigate roles of the miRNA pathway genes in MZT in the Drosophila embryo, we generated several GFP knocked-in flies for Dcr-1, Ago1 and Gw. We knocked down these GFP fusion protein specilicaally in early embryogenesis by activating deGradFP system, which poly-ubiquitinates GFP-fusion proteins to promote their degradation. We found that when GFP-tagged Gw was knocked down, about 50% of embryos stalled embryogenesis at the stage during which the MZT starts, suggesting that the miRNA pathway is indeed involved in the MZT.
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