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Investigation roles of the miRNA pathway in maternal-to-xygotic transition

Research Project

Project/Area Number 15K14530
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionKumamoto University

Principal Investigator

Nakamura Akira  熊本大学, 発生医学研究所, 教授 (90323245)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsショウジョウバエ / CRISPR-Cas9 / miRNA / ゲノム編集 / 母性-胚性遷移 / マイクロRNA
Outline of Final Research Achievements

The maternal-to-Zygotic transition (MZT) involves degradation of maternal product to switch zygotic gene-oriented development, and is the conserved process in animal development. To investigate roles of the miRNA pathway genes in MZT in the Drosophila embryo, we generated several GFP knocked-in flies for Dcr-1, Ago1 and Gw. We knocked down these GFP fusion protein specilicaally in early embryogenesis by activating deGradFP system, which poly-ubiquitinates GFP-fusion proteins to promote their degradation. We found that when GFP-tagged Gw was knocked down, about 50% of embryos stalled embryogenesis at the stage during which the MZT starts, suggesting that the miRNA pathway is indeed involved in the MZT.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 2015 Other

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] The evolutionally-conserved function of group B1 Sox family members confers the unique role of Sox2 in mouse ES cells2016

    • Author(s)
      2.Niwa, H., Nakamura, A., Urata, M., Shirae-Kurabayashi, M., Kuraku, S. and Ohtsuka, S.
    • Journal Title

      BMC Evol. Biol.

      Volume: 16 Issue: 1 Pages: 173-173

    • DOI

      10.1186/s12862-016-0755-4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The nutrient-responsive hormone CCHamide-2 controls growth by regulating insulin-like peptides in the brain of Drosophila melanogaster2015

    • Author(s)
      Sano H, Nakamur A, Texada M, Truma J, Ishimoto H, Kamikouchi A, Nibu Y, Kume K, Ida T, Kojima M
    • Journal Title

      PLoS Genetics

      Volume: 11 Issue: 5 Pages: 1-26

    • DOI

      10.1371/journal.pgen.1005209

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Efficient endocytic uptake and maturation in Drosophila oocytes requires Dynamitin/p50.2015

    • Author(s)
      Liu, G., Sanghavi, P., Bollinger, K. E., Perry, L., Marshall, B., Roon, P., Tanaka, T., Nakamura, A. and Gonsalvez, G. B.
    • Journal Title

      Genetics

      Volume: 201 Issue: 2 Pages: 631-649

    • DOI

      10.1534/genetics.115.180018

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ショウジョウバエの小胞型H/Cl交換輸送体Clc-bは長鎖dsRNAを介したRNAi誘導に必要である2017

    • Author(s)
      田中翼、植田忠大、大津佐千子、反町典子、中村輝、Katija Bruckner
    • Organizer
      第17回日本RNA学会年会
    • Place of Presentation
      ホテルライフォート札幌
    • Year and Date
      2017-07-15
    • Related Report
      2015 Research-status Report
  • [Presentation] A novel genetic strategy to investigate embryonic roles of maternal factors with essential functions in oogenesis2016

    • Author(s)
      Nakamura, A. and Yoshitani, T.
    • Organizer
      12th Japanese Drosophila Research Conference
    • Place of Presentation
      立教大学
    • Year and Date
      2016-09-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] An oogenic stage-specific RNAi screening for novel maternal-effect genes regulating germ cell development in the Drosophila embryo2016

    • Author(s)
      Aimi, K., Hanyu-nakamura, K., and Nakamura, A.
    • Organizer
      12th Japanese Drosophila Research Conference
    • Place of Presentation
      立教大学
    • Year and Date
      2016-09-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] OOcyte polarity establishment and germ plasm assembly require the endocytic regulation of the yolk protein receptor Yolkless2016

    • Author(s)
      Tanaka, T., Otsu, S., Tani, N., and Nakamura, A.
    • Organizer
      12th Japanese Drosophila Research Conference
    • Place of Presentation
      立教大学
    • Year and Date
      2016-09-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規の遺伝学ツールを用いたショウジョウバエ初期胚におけるRNAサイレンシング因子の機能解析2015

    • Author(s)
      中村 輝
    • Organizer
      第17回日本RNA学会年会
    • Place of Presentation
      ホテルライフォート札幌
    • Year and Date
      2015-07-15
    • Related Report
      2015 Research-status Report
  • [Presentation] The nutrient-responsive hormone CCHamide-2 controls growth by regulating insulin-like peptides in the brain of Drosophila melanogaster.2015

    • Author(s)
      Sano, H., Nakamura, A., Texada, M., Truman, J. W., Ishimoto, H., Kamikouchi, A., Nibu, Y., Kume, K., Ida, T. and Kojima, M.
    • Organizer
      第48回日本発生生物学会年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-06-02
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] 熊本大学発生医学研究所 研究室紹介ページ

    • URL

      http://www.imeg.kumamoto-u.ac.jp/bunya_top/germline_development/

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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