| Project/Area Number |
15K14557
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Saitama University |
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Principal Investigator |
SAKATA Ichiro 埼玉大学, 理工学研究科, 准教授 (80610831)
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| Co-Investigator(Kenkyū-buntansha) |
坂井 貴文 埼玉大学, 理工学研究科, 教授 (40235114)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | グレリン / 側鎖修飾 / 長鎖脂肪酸 / 中鎖脂肪酸 / β酸化 / アシル化 / GOAT / ghrelin / 脂肪酸 |
|---|
| Outline of Final Research Achievements |
Ghrelin is 28-amino acids peptide hormone and Ser3 of ghrelin is modified by medium-chain fatty acid (MCFA). This modification is essential for ghrelin activities. We examined three possibilities of the MCFA origin; diet, intestinal bacteria and de novo synthesis using mice and ghrelinoma cell lines (PG-1 cell). We found that a fat-free diet and the removal of intestinal microbiota did not decrease acyl-ghrelin production in the stomach or plasma acyl-ghrelin levels in mice. Blockage of β-oxidation in PG-1 cells strongly decreased acylated ghrelin levels. These results suggest the medium-chain fatty acid used for the acyl-modification of ghrelin is produced in ghrelin-producing cells themselves by β-oxidation of long-chain fatty acids.
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