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Study on a non-visual photoreception lost in the course of mammalian evolution

Research Project

Project/Area Number 15K14568
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Animal physiology/Animal behavior
Research InstitutionOsaka City University

Principal Investigator

Koyanagi Mitsumasa  大阪市立大学, 大学院理学研究科, 准教授 (30379276)

Co-Investigator(Renkei-kenkyūsha) TERAKITA Akihisa  大阪市立大学, 大学院理学研究科, 教授 (30212062)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords進化 / 光感覚 / 有袋類 / 生理学 / ロドプシン
Outline of Final Research Achievements

We discovered a photoreceptor gene (opsin), which does not exist in eutherian genomes, in marsupial genomes. In this study, we carried out functional analyses of the marsupial opsin spectroscopically and biochemically to understand the diversity of photoreception in mammals. We found that the marsupial opsin indeed forms a photosensitive pigment. In addition, histochemical analysis revealed that the marsupial opsin exhibits a different expression pattern from other opsins in opossum, suggesting a novel photoreception in mammals.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (41 results)

All 2017 2016 2015 Other

All Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (32 results) (of which Int'l Joint Research: 13 results,  Invited: 5 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Absorption Characteristics of Vertebrate Non-Visual Opsin, Opn32016

    • Author(s)
      T. Sugihara, T. Nagata, B. Mason, M. Koyanagi and A. Terakita
    • Journal Title

      PLOS ONE

      Volume: 11 Issue: 8 Pages: e0161215

    • DOI

      10.1371/journal.pone.0161215

    • NAID

      120006345645

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2020440

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Vertebrate Bistable Pigment Parapinopsin: Implications for Emergence of Visual Signaling and Neofunctionalization of Non-visual Pigment.2016

    • Author(s)
      M. Koyanagi, E. Kawano-Yamashita, S. Wada and A. Terakita
    • Journal Title

      Frontiers in Ecology and Evolution

      Volume: 5 Pages: 23-23

    • DOI

      10.3389/fevo.2017.00023

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Diversification of non-visual photopigment parapinopsin in spectral sensitivity for diverse pineal functions2015

    • Author(s)
      M. Koyanagi, S. Wada, E. Kawano-Yamashita, Y. Hara, S. Kuraku, S. Kosaka, K. Kawakami, S. Tamotsu, H. Tsukamoto, Y. Shichida, A. Terakita
    • Journal Title

      BMC Biology

      Volume: 13 Issue: 1 Pages: 73-73

    • DOI

      10.1186/s12915-015-0174-9

    • NAID

      120005758203

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Activation of Transducin by Bistable Pigment Parapinopsin in the Pineal Organ of Lower Vertebrates2015

    • Author(s)
      E. Kawano-Yamashita, M. Koyanagi, S. Wada, H. Tsukamoto, T. Nagata, A. Terakita
    • Journal Title

      PLOS ONE

      Volume: 10 Issue: 10 Pages: e0141280

    • DOI

      10.1371/journal.pone.0141280

    • NAID

      120006346629

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2020439

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Retinal attachment instability is diversified among mammalian melanopsins2015

    • Author(s)
      H. Tsukamoto, Y. Kubo, D.L. Farrens, M. Koyanagi, A. Terakita, Y. Furutani
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 290 Issue: 45 Pages: 27176-27187

    • DOI

      10.1074/jbc.m115.666305

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Light-dependent interaction of bistable opsin-based pigments with arrestin2017

    • Author(s)
      T. Nagata, M. Koyanagi, E. Kawano-Yamashita, R. Lucas, A. Terakita
    • Organizer
      8th OCARINA International Symposium
    • Place of Presentation
      大阪市立大学(大阪府・大阪市)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular and cellular mechanisms of the extraocular "color" discrimination in the lower vertebrates2017

    • Author(s)
      S. Wada, E. Kawano-Yamashita, S. Tamotsu, M. Koyanagi, A. Terakita
    • Organizer
      8th OCARINA International Symposium
    • Place of Presentation
      大阪市立大学(大阪府・大阪市)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular properties of vertebrate non-visual opsin, Opn32017

    • Author(s)
      T. Sugihara, T. Nagata, B. Mason, M. Koyanagi, A. Terakita
    • Organizer
      8th OCARINA International Symposium
    • Place of Presentation
      大阪市立大学(大阪府・大阪市)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation of optogenetic potentials of a non-visual opsin, Mosquito Opn32017

    • Author(s)
      B. Shen, M. Koyanagi, L. Sun, S. Wada, T. Nagata, S. Kamimura, E. Kage-Nakadai, A. Terakita
    • Organizer
      8th OCARINA International Symposium
    • Place of Presentation
      大阪市立大学(大阪府・大阪市)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optogenetic potentials of bistable animal opsin-based pigments for regulating GPCR signalings2016

    • Author(s)
      小柳光正
    • Organizer
      第54回生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県・つくば市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Light-dependent association and dissociation of arrestin with bistable opsins2016

    • Author(s)
      永田崇、小柳光正、山下(川野)絵美、Robert Lucas、寺北明久
    • Organizer
      第54回生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県・つくば市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] Potential of bistable pigments for optical control of cell signaling2016

    • Author(s)
      M. Koyanagi
    • Organizer
      22nd International Congress of Zoology & 87th meeting of Zoological Society of Japan
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-11-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Spectral sensitivities and spectral tuning of butterfly long-wavelength-sensitive opsins2016

    • Author(s)
      M. Koyanagi, T. Saito, T. Sugihara, K. Arikawa, A. Terakita
    • Organizer
      22nd International Congress of Zoology & 87th meeting of Zoological Society of Japan
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-11-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparative investigation of molecular basis of pineal wavelength discrimination in lower vertebrates2016

    • Author(s)
      S. Wada, E. Kawano-Yamashita, S. Tamotsu, M. Koyanagi, A. Terakita
    • Organizer
      22nd International Congress of Zoology & 87th meeting of Zoological Society of Japan
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-11-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of neural connection between pineal organ and some brain regions in zebrafish2016

    • Author(s)
      M. Kakiya, S. Wada, E. Kawano-Yamashita, M. Koyanagi, A. Terakita
    • Organizer
      22nd International Congress of Zoology & 87th meeting of Zoological Society of Japan
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-11-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optical control of cell signaling using bistable animal opsin-based pigments2016

    • Author(s)
      M. Koyanagi
    • Organizer
      17th International Conference on Retinal Proteins
    • Place of Presentation
      Potsdam (Germany)
    • Year and Date
      2016-10-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular characteristics of vertebrate non-visual opsin, Opn32016

    • Author(s)
      T. Sugihara, T. Nagata, B. Mason, M. Koyanagi, A. Terakita
    • Organizer
      17th International Conference on Retinal Proteins
    • Place of Presentation
      Potsdam (Germany)
    • Year and Date
      2016-10-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The molecular and cellular basis of the wavelength discrimination in the zebrafish pineal organ2016

    • Author(s)
      S. Wada, E. Kawano-Yamashita, S. Tamotsu, M. Koyanagi, A. Terakita
    • Organizer
      17th International Conference on Retinal Proteins
    • Place of Presentation
      Potsdam (Germany)
    • Year and Date
      2016-10-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spectral and biochemical characterization of mammalian melanopsins (Opn4s) and invertebrate Opn3 homologs2016

    • Author(s)
      H. Tsukamoto, Y. Kubo, D.L. Farrens, M. Koyanagi, A. Terakita, Y. Furutani
    • Organizer
      17th International Conference on Retinal Proteins
    • Place of Presentation
      Potsdam (Germany)
    • Year and Date
      2016-10-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 硬骨魚類松果体の波長依存的な拮抗反応に関わる光受容細胞の解析2016

    • Author(s)
      和田清二、山下(川野)絵美、保智己、小柳光正、寺北明久
    • Organizer
      第19回日本光生物学協会年会
    • Place of Presentation
      東京大学(東京都・文京区)
    • Year and Date
      2016-07-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脊椎動物が持つ非視覚オプシンOpn3の培養細胞での発現と分子特性の解析2016

    • Author(s)
      杉原智博、永田崇、Benjamin Mason、小柳光正、寺北明久
    • Organizer
      第19回日本光生物学協会年会
    • Place of Presentation
      東京大学(東京都・文京区)
    • Year and Date
      2016-07-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] ナミアゲハ赤感受性オプシンの吸収波長制御機構の解析2016

    • Author(s)
      小柳光正、斉藤智香、杉原智博、蟻川謙太郎、寺北明久
    • Organizer
      第19回日本光生物学協会年会
    • Place of Presentation
      東京大学(東京都・文京区)
    • Year and Date
      2016-07-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 非視覚オプシンであるハマダラカOpn3の光遺伝学的利用の可能性の検討2016

    • Author(s)
      沈宝國、小柳光正、孫蘭芳、和田清二、永田崇、上村了美、中田(鹿毛)枝里子、寺北明久
    • Organizer
      第19回日本光生物学協会年会
    • Place of Presentation
      東京大学(東京都・文京区)
    • Year and Date
      2016-07-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] ゼブラフィッシュ網膜におけるメラノプシン発現水平細胞の電気生理学的、組織学的解析2016

    • Author(s)
      乾寛、西村充、和田清二、小柳光正、寺北明久
    • Organizer
      第19回日本光生物学協会年会
    • Place of Presentation
      東京大学(東京都・文京区)
    • Year and Date
      2016-07-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 哺乳類における「非視覚」の光受容機能を支えるメラノプシンの分子基盤2016

    • Author(s)
      塚本寿夫、久保 義弘、David Farrens、小柳光正、寺北明久、古谷 祐詞
    • Organizer
      第43回生体分子科学討論会
    • Place of Presentation
      名古屋大学(愛知県・名古屋市)
    • Year and Date
      2016-06-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Characterization of opsin-based pigments by bioluminescence monitoring of second-messengers in cultured cells2015

    • Author(s)
      Mitsumasa Koyanagi, Tomohiro Sugihara, Takashi Nagata, Kentaro Arikawa, Akihisa Terakita
    • Organizer
      第40回日本比較内分泌学会大会・日本比較生理生化学会第37回大会 合同大会(CompBiol 2015 広島大会)
    • Place of Presentation
      JMSアステールプラザ(広島県・広島市)
    • Year and Date
      2015-12-12
    • Related Report
      2015 Research-status Report
  • [Presentation] Electrophysiological investigation of melanopsin-expressing retinal cells in the zebrafish retina2015

    • Author(s)
      Hiroshi Inui, Mitsumasa Koyanagi, Akihisa Terakita
    • Organizer
      第40回日本比較内分泌学会大会・日本比較生理生化学会第37回大会 合同大会(CompBiol 2015 広島大会)
    • Place of Presentation
      JMSアステールプラザ(広島県・広島市)
    • Year and Date
      2015-12-12
    • Related Report
      2015 Research-status Report
  • [Presentation] Molecular basis of wavelength discrimination in the lamprey pineal organ2015

    • Author(s)
      Seiji Wada, Emi Kawano-Yamashita, Satoshi Tamotsu, Mitsumasa Koyanagi, Akihisa Terakita
    • Organizer
      第40回日本比較内分泌学会大会・日本比較生理生化学会第37回大会 合同大会(CompBiol 2015 広島大会)
    • Place of Presentation
      JMSアステールプラザ(広島県・広島市)
    • Year and Date
      2015-12-12
    • Related Report
      2015 Research-status Report
  • [Presentation] 光受容タンパク質の意外な使い方~ピンぼけ像を使った奥行き知覚~2015

    • Author(s)
      小柳光正
    • Organizer
      第49回光学五学会関西支部連合講演会
    • Place of Presentation
      大阪市立大学文化交流センター(大阪府・大阪市)
    • Year and Date
      2015-12-05
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 非視覚型光受容タンパク質Opn3グループの多様性の解析2015

    • Author(s)
      小柳光正、杉原智博、永田崇、寺北明久
    • Organizer
      日本動物学会第86回大会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-19
    • Related Report
      2015 Research-status Report
  • [Presentation] 遺伝子導入ゼブラフィ ッシュを用いたメラノプシン発現水平細胞の解析2015

    • Author(s)
      西村充,小柳光正,寺北明久
    • Organizer
      日本動物学会第86回大会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-19
    • Related Report
      2015 Research-status Report
  • [Presentation] 円口類松果体の波長依存的な拮抗反応を生み出すメカニズムの解析2015

    • Author(s)
      和田清二,山下(川野)絵美,保智己,小柳光正,寺北明久
    • Organizer
      日本動物学会第86回大会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-19
    • Related Report
      2015 Research-status Report
  • [Presentation] ゼブラフィッシュ副松果体におけるパラピノプシン発現細胞の組織学的解析2015

    • Author(s)
      山下(川野)絵美,和田清二,小柳光正,寺北明久
    • Organizer
      日本動物学会第86回大会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-19
    • Related Report
      2015 Research-status Report
  • [Presentation] ナメクジウオの内柱における糖タンパク質ホルモンの存在2015

    • Author(s)
      窪川かおる,小柳光正,小笠原道生,石黒宗,程久美子,寺北明久
    • Organizer
      日本動物学会第86回大会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-19
    • Related Report
      2015 Research-status Report
  • [Presentation] Estimating spectral sensitivities of non-visual opsins based on biochemical responses of opsin-expressing cultured cells2015

    • Author(s)
      杉原智博、永田崇、小柳光正、寺北明久
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県・金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] Introduction of animal rhodopsins and their optogenetic potentials2015

    • Author(s)
      Mitsumasa Koyanagi
    • Organizer
      光化学討論会
    • Place of Presentation
      大阪市立大学(大阪府・大阪市)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Activation of Transducin by Bistable Pigment Parapinopsin in the Pineal Organ of Lower Vertebrates2015

    • Author(s)
      Mitsumasa Koyanagi, Emi Kawano-Yamashita, Seiji Wada, Takashi Nagata, Akihisa Terakita
    • Organizer
      FASEB SUMMER RESEARCH CONFERENCES
    • Place of Presentation
      Montana (USA)
    • Year and Date
      2015-06-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] 光と生命の事典2016

    • Author(s)
      小柳光正
    • Total Pages
      436
    • Publisher
      朝倉書店
    • Related Report
      2016 Annual Research Report
  • [Book] Optogenetics2015

    • Author(s)
      A. Terakita, T. Nagata, T. Sugihara, M. Koyanagi
    • Total Pages
      409
    • Publisher
      Springer
    • Related Report
      2015 Research-status Report
  • [Remarks] 生体高分子機能学II研究室

    • URL

      http://www.sci.osaka-cu.ac.jp/biol/mphys/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 生体高分子機能学Ⅱ研究室

    • URL

      http://www.sci.osaka-cu.ac.jp/biol/mphys/index.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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