| Project/Area Number |
15K14576
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 単為発生 / 中心体 / ゲノム / 受精 / 接合子 / ショウジョウバエ / 単為生殖 / 紡錘体 / 未受精卵 / 減数分裂 |
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| Outline of Final Research Achievements |
The genetic changes that allow the transition from sexual reproduction to parthenogenesis in animals remain elusive. To address the problem we here performed the following experiments using Drosophila melanogaster and D. ananassae. Firstly, we tried to induce parthenogenenetic development of D. melanogaster unfertilized eggs, with de novo formation of centrosomes in the egg cytoplasm. Secondly, our comparative genomic analysis of the sexual and parthenogenetic strains of D. ananassae identified two genomic regions on the second chromosome essential for parthenogenetic development to occur. Finally, we have shown that the maternal-effect gene Elys of D. melanogaster is required for zygote formation using the two nuclei from the ovum and sperm.
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