Functional analysis of cyanobacterial rhodopsins: How do they use the two different phototrophic systems.
| Project/Area Number |
15K14601
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ecology/Environment
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ロドプシン / シアノバクテリア / バクテリア / 微生物生態 / 光合成 / 海洋細菌 / 海洋生態 / 細菌 / ゲノム / 系統進化 |
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| Outline of Final Research Achievements |
A novel rhodopsin was discovered from cyanobacteria (Synechocystis sp. PCC 7509) and named SyHR. Functional analysis showed that SyHR functions as light-driven inward anion (Cl- and SO42-) pump. Spectroscopic analysis revealed that SyHR utilize green light (about 540 nm). These results showed that cyanobacterial SyHR actively transports anion using green light not absorbed by chlorophyll. Also, the rhodopsin which transports divalent anions has not been found so far, and a first light-driven divalent anion pump was shown in this study.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Demonstration of a light-driven SO42- transporter and its spectroscopic characteristics2017
Author(s)
Niho A, Yoshizawa S, Tsukamoto T, Kurihara M, Tahara S, Nakajima Y, Mizuno M, Kuramochi H, Tahara T, Mizutani Y, *Sudo Y.
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Journal Title
J. Am. Chem. Soc.
Volume: 139
Issue: 12
Pages: 4376-4389
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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