Functional analysis of cyanobacterial rhodopsins: How do they use the two different phototrophic systems.

• Project/Area Number: 15K14601
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: The University of Tokyo
• Principal Investigator: Yoshizawa Susumu 東京大学, 大気海洋研究所, 准教授 (00553108)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ロドプシン / シアノバクテリア / バクテリア / 微生物生態 / 光合成 / 海洋細菌 / 海洋生態 / 細菌 / ゲノム / 系統進化

Outline of Final Research Achievements

A novel rhodopsin was discovered from cyanobacteria (Synechocystis sp. PCC 7509) and named SyHR. Functional analysis showed that SyHR functions as light-driven inward anion (Cl- and SO42-) pump. Spectroscopic analysis revealed that SyHR utilize green light (about 540 nm). These results showed that cyanobacterial SyHR actively transports anion using green light not absorbed by chlorophyll. Also, the rhodopsin which transports divalent anions has not been found so far, and a first light-driven divalent anion pump was shown in this study.

Research Products

• [Journal Article] Presence of a Haloarchaeal Halorhodopsin-Like Cl⁻ Pump in Marine Bacteria (2018)
  • Author(s): Nakajima Yu、Tsukamoto Takashi、Kumagai Yohei、Ogura Yoshitoshi、Hayashi Tetsuya、Song Jaeho、Kikukawa Takashi、Demura Makoto、Kogure Kazuhiro、Sudo Yuki、Yoshizawa Susumu
  • Journal Title: Microbes and Environments Volume: 33 Issue: 1 Pages: 89-97
  • DOI: 10.1264/jsme2.ME17197
  • NAID: 130006581777
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Draft Genome Sequence of Rubricoccus marinus SG-29 T , a Marine Bacterium within the Family Rhodothermaceae , Which Contains Two Different Rhodopsin Genes (2017)
  • Author(s): Nakajima Yu、Yoshizawa Susumu、Park Sanghwa、Kumagai Yohei、Wong Shu-Kuan、Ogura Yoshitoshi、Hayashi Tetsuya、Kogure Kazuhiro
  • Journal Title: Genome Announcements Volume: 5 Issue: 38
  • DOI: 10.1128/genomea.00990-17
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Demonstration of a light-driven SO42- transporter and its spectroscopic characteristics (2017)
  • Author(s): Niho A, Yoshizawa S, Tsukamoto T, Kurihara M, Tahara S, Nakajima Y, Mizuno M, Kuramochi H, Tahara T, Mizutani Y, *Sudo Y.
  • Journal Title: J. Am. Chem. Soc. Volume: 139 Issue: 12 Pages: 4376-4389
  • DOI: 10.1021/jacs.6b12139
  • NAID: 120006425837
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] ロドプシン保持細菌の例から考える、光従属栄養性のメリットとデメリット (2018)
  • Author(s): 吉澤 晋
  • Organizer: 第３回光合成細菌ワークショップ
  • Invited
• [Presentation] シアノバクテリアの持つ光駆動型イオン輸送ロドプシン-彼らはどのような環境で、どのイオンを輸送しているのか？- (2016)
  • Author(s): 吉澤 晋
  • Organizer: ラン藻ゲノム交流会
  • Place of Presentation: 東京大学駒場キャンパス１６号館（東京都・目黒区）
  • Year and Date: 2016-06-25
  • Invited
• [Presentation] ロドプシンで太陽エネルギーを利用する-光駆動型イオンポンプ- (2016)
  • Author(s): 吉澤晋
  • Organizer: 第８９回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター （大阪府・大阪市）
  • Year and Date: 2016-03-23
  • Invited
• [Presentation] 淡水性シアノバクテリアの持つ光駆動型陰イオン取り込みロドプシン-彼らは淡水環境でどのイオンを輸送しているのか？- (2015)
  • Author(s): 吉澤晋
  • Organizer: 藍藻の分子生物学2015
  • Place of Presentation: かずさアカデミアホール （千葉県・木更津市）
  • Year and Date: 2015-11-16
• [Presentation] 「光合成」と「ロドプシン」、二つの異なる光利用システムを繋げる未知遺伝子DUF2237の機能解析 (2015)
  • Author(s): 熊谷洋平, 吉澤晋, 渡辺麻衣, 池内昌彦, 木暮一啓, 岩崎渉
  • Organizer: 藍藻の分子生物学2015
  • Place of Presentation: かずさアカデミアホール （千葉県・木更津市）
  • Year and Date: 2015-11-16
• [Presentation] unctional studies on a light-driven anion pump from Synechocystis sp. PCC 7509 (2015)
  • Author(s): 仁保 亜紀子, 吉澤 晋, 中島 悠, 塚本 卓, 須藤 雄気
  • Organizer: 第５３回日本生物物理学会年会
  • Place of Presentation: 金沢大学角間キャンパス 自然科学本館 （石川県・金沢市）
  • Year and Date: 2015-09-13