| Project/Area Number |
15K14627
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Ishikawa Prefectural University |
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Principal Investigator |
Hamada Tatsuro 石川県立大学, 生物資源環境学部, 准教授 (50310496)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | イネ / アスパラギン酸プロテアーゼ / タンパク質 / 窒素源 / 形質転換体 / 根圏 |
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| Outline of Final Research Achievements |
Transgenic rice overexpressing aspartic protease gene (OsASP2) and nontransformed rice were cultivated in MS and MS-IN, casein media for 30 days, and the growth of both were compared. In MS medium, no significant growth difference was found between them. On the other hand, in MS-IN, casein medium, leaf length and dry weight of the OsASP2 transgenic rice were about 3.7 and 1.5 times those of the non-transformant, respectively. This result revealed that the release of aspartic protease from the root to the rhizosphere in the transgenic rice contributed to the use of protein as a nitrogen source for growth.
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