Development of rice with enhanced nitrogen acquisition ability from soil

Research Project

Project/Area Number	15K14627
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Science in genetics and breeding
Research Institution	Ishikawa Prefectural University
Principal Investigator	Hamada Tatsuro 石川県立大学, 生物資源環境学部, 准教授 (50310496)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000) Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	イネ / アスパラギン酸プロテアーゼ / タンパク質 / 窒素源 / 形質転換体 / 根圏
Outline of Final Research Achievements	Transgenic rice overexpressing aspartic protease gene (OsASP2) and nontransformed rice were cultivated in MS and MS-IN, casein media for 30 days, and the growth of both were compared. In MS medium, no significant growth difference was found between them. On the other hand, in MS-IN, casein medium, leaf length and dry weight of the OsASP2 transgenic rice were about 3.7 and 1.5 times those of the non-transformant, respectively. This result revealed that the release of aspartic protease from the root to the rhizosphere in the transgenic rice contributed to the use of protein as a nitrogen source for growth.