Fungistatic activity of bacteria isolated from a novel hydroponics system

• Project/Area Number: 15K14666
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Iida Yuichiro 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門・野菜病害虫・機能解析研究領域, 主任研究員 (00456609)
• Co-Investigator(Kenkyū-buntansha): 篠原 信 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門 野菜病害虫・機能解析研究領域, 上級研究員 (90326075)
• Research Collaborator: FUJIWARA Kazuki 国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター 生産環境研究領域, 任期付研究員 (40725008) ; SOMEYA Nobutaka 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門 野菜生産システム研究領域, 上級研究員 (60360575)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 有機質肥料活用型養液栽培 / Fusarium oyxsporum / Fusarium oxysporum / Rhizobium sp. / III型分泌装置 / 微生物間相互作用

Outline of Final Research Achievements

Multiple parallel mineralization (MPM) is a novel hydroponics system in which soil microorganisms mineralize organic fertilizers in a hydroponic solution, supplying nitrate nitrogen to plants. The MPM solution exhibits operative suppressiveness of both fungal and bacterial root-borne diseases, similar to natural disease-suppressive soils. The microbial community is able to inhibit mycelial growth and chlamydospore germination of plant pathogenic fungus Fusarium oxysporum, but does not eliminate the pathogen (showing fungistatic activity). In this study, we investigated which members of the MPM microbiota contribute to root disease suppression, and analyzed the mechanisms for its fungistatic activity.

Research Products

• [Journal Article] Draft genome sequence of Rhizobium sp. strain TBD-182, an antagonist of the plant pathogenic fungus Fusarium oxysporum, isolated from a novel hydroponics system using organic fertilizer (2017)
  • Author(s): Yuichiro Iida, Kazuki Fujiwara, Nobutaka Someya, Makoto Shinohara
  • Journal Title: Genome announcement Volume: 5 Issue: 11
  • DOI: 10.1128/genomea.00007-17
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Emergence of antagonism against the pathogenic fungus Fusarium oxysporum by interplay among non-antagonistic bacteria in a hydroponics using multiple parallel mineralization (2016)
  • Author(s): Kazuki Fujiwara, Yuichiro Iida, Nobutaka Someya, Masao Takano, Jun Ohnishi, Fumihiro Terami, Makoto Shinohara
  • Journal Title: Journal of Phytopathology Volume: 164 Issue: 11-12 Pages: 853-862
  • DOI: 10.1111/jph.12504
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Synergistic action of serine- and metallo-proteases from Fusarium oxysporum f. sp. lycopersici cleaves chitin-binding tomato chitinases, reduces their antifungal activity and enhances fungal virulence (2015)
  • Author(s): Mansoor Karimi Jashni, Ivo Dols, Yuichiro Iida, Sjef Boeren, Henriek G. Beenen, Rahim Mehrabi, Jérôme Collemare, Todd Naumann, Pierre J. G. M. de Wit
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 9 Issue: 9 Pages: 996-1008
  • DOI: 10.1094/mpmi-04-15-0074-r
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Fusarium oxysporumの菌糸伸張を抑制する土壌細菌Rhizobium sp. (2015)
  • Author(s): 飯田祐一郎, 藤原和樹, 篠原信, 渡辺郁栄
  • Organizer: フザリウム研究会
  • Place of Presentation: 三重県伊勢市、神宮会館
  • Year and Date: 2015-08-28