| Project/Area Number |
15K14713
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagaoka National College of Technology |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ミミズ / 形質転換法 / モデル生物 / バイオ医薬品 / 物質生産 |
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| Outline of Final Research Achievements |
We are attempting to develop genetic engineering techniques of earthworm, Eisenia fetida, for producing biological medicines. Although we have succeeded to construct E. fetida transformation method using posterior of the earthworm, the survival rate and transformation efficiency are very low. Thus, we attempted to improve these rates. As a result, the survival rate and transformation efficiency were improved to 5.9- and 1.5-fold, respectively. These results will lead to the development of complete earthworm recombinant.
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