| Project/Area Number |
15K14758
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Forest science
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| Research Institution | Kyushu University |
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Principal Investigator |
Shiraishi Susumu 九州大学, (連合)農学研究科(研究院), 教授 (70226314)
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| Co-Investigator(Kenkyū-buntansha) |
Gotoh Eiji 九州大学, 大学院農学研究院, 助教 (90614256)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | DNA分子マーカー / 葉緑体ゲノム / １塩基マイクロサテライト / ハプロタイプ / 裸子植物 / ハプロタイピング |
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| Outline of Final Research Achievements |
In forest genetics, breeding and ecology, chloroplast DNA markers are essential as paternal markers, since chloroplast genome is inherited from only its paternal parent. Most of the DNA markers, however, are single nucleotide SSR (homopolymer). Therefore, an analysis method (HoPPE：homopolymer primer extension) was newly developed, that is able to detect precisely the variations of single nucleotide repeats in the homopolymer regions.
A computer program for translating the HoPPE data to DNA types was created using programming macros in Excel (Microsoft).
In Japanese cedar (Sugi), the frequencies of homopolymer regions in the chloroplast genome were estimated for validating the homopolymer polymorphism.
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