Stable isotope analysis of epidermal mucus to estimate food habits and migration without time lag

• Project/Area Number: 15K14792
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Ryukoku University
• Principal Investigator: Maruyama Atsushi 龍谷大学, 理工学部, 准教授 (70368033)
• Co-Investigator(Renkei-kenkyūsha): YONEKURA Ryuji 岐阜県水産研究所, 研究員 (40455514)
• Research Collaborator: SHIGETA Kanji ; SAWADA Hayato ; UEDA Yoshiki ; HIRAISHI Yumiko
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 安定同位体 / 粘液 / 置換速度 / 濃縮係数 / 淡水魚類 / 魚類 / 安定同位体比

Outline of Final Research Achievements

The goal of this project was to establish stable isotope analysis of fish epidermal mucus to estimate food habits and migration history without tame lags. We measured trophic discrimination factors and turnover rates of epidermal mucus of several freshwater fish species by experiments and showed that mucus isotopes turnover more quickly than widely-used muscle tissues, and that epidermal mucus can be repeatedly collected from living fish. In addition, we found that the growth and catabolic turnover rates correlate with each other, which may enable estimation of isotopic change of quickly-responding mucus in the field, where growth rates are only available. Thus, we propose the use of fish epidermal mucus for the isotope studies which require quick response or non-lethal sample collections.

Research Products

• [Journal Article] Species-specific detection of the endangered piscivorous cyprinid fish Opsariichthys uncirostris uncirostris, three-lips, using environmental DNA analysis (2018)
  • Author(s): Yamanaka Hiroki、Takao Daiki、Maruyama Atsushi、Imamura Akio
  • Journal Title: Ecological Research Volume: － Issue: 5 Pages: 1075-1078
  • DOI: 10.1007/s11284-018-1612-2
  • NAID: 40021665106
  • Peer Reviewed
• [Journal Article] Examination of trophic discrimination factors and lipid corrections in muscle, ovary and mucus tissues of ayu (<i>Plecoglossus altivelis altivelis</i>) using carbon and nitrogen stable isotope analyses (2018)
  • Author(s): 沢田 隼、米倉 竜次、丸山 敦
  • Journal Title: Japanese Journal of Ichthyology Volume: 65 Issue: 1 Pages: 1-7
  • DOI: 10.11369/jji.17-017
  • NAID: 130006726275
  • ISSN: 0021-5090, 1884-7374
  • Peer Reviewed
• [Journal Article] Isotopic analysis of epidermal mucus in freshwater fishes can reveal short-time diet variations (2017)
  • Author(s): Shigeta Kanji、Tsuma Shiori、Yonekura Ryuji、Kakamu Hiroto、Maruyama Atsushi
  • Journal Title: Ecological Research Volume: 32 Issue: 5 Pages: 643-652
  • DOI: 10.1007/s11284-017-1478-8
  • NAID: 40021323433
  • Peer Reviewed
• [Journal Article] Functions of indigenous animals in paddy fields: an in situ experiment on their effects on water quality, phytoplankton, weeds, soil structure, and rice growth (2017)
  • Author(s): Iwai Noriko、Koyama Nana、Tsuji Sakie、Maruyama Atsushi
  • Journal Title: Paddy and Water Environment Volume: 16 Issue: 1 Pages: 89-98
  • DOI: 10.1007/s10333-017-0618-7
  • Peer Reviewed
• [Journal Article] Empty gut ratio and body shape of the three-lips (<i>Opsariichthys uncirostris uncirostris</i>) at the shoreline of Lake Biwa in summer 2015 (2017)
  • Author(s): 今村 彰生、橋本 果穂、丸山 敦
  • Journal Title: Izunuma-Uchinuma Wetland Researches Volume: 11 Issue: 0 Pages: 29-40
  • DOI: 10.20745/izu.11.0_29
  • NAID: 130006199866
  • ISSN: 1881-9559, 2424-2101
  • Peer Reviewed
• [Journal Article] ニジマスの採餌応答に見られるルアー色と濁度の交互作用 (2017)
  • Author(s): 西大嵩樹, 丸山 敦
  • Journal Title: 魚類学雑誌 Volume: 64 Pages: 113-119
  • NAID: 130007397859
• [Journal Article] 変態の遅延するモリアオガエル (Rhacophorus arboreus) 幼生の観察および炭素・窒素安定同位体分析による生態的地位の推定 (2017)
  • Author(s): 神松幸弘, 丸山 敦
  • Journal Title: 爬虫両棲類学会誌 Volume: 印刷中
  • NAID: 40021240372
  • Peer Reviewed
• [Journal Article] メコン川下流水田域における生業、土地利用、生態系サービス：水位変動下における適応 (2017)
  • Author(s): 神松幸弘, 富田晋介, 丸山 敦, 船津耕平, 門司和彦
  • Journal Title: 環太平洋文明研究 Volume: 1 Pages: 69-92
  • NAID: 40021803373
• [Journal Article] A comparison of changes in stable isotope ratios in the epidermal mucus and muscle tissue of slow-growing adult catfish. (2016)
  • Author(s): Maruyama, A., Tanahashi, E., Hirayama, T., and Yonekura, R
  • Journal Title: Ecology of Freshwater Fish Volume: － Issue: 4 Pages: 636-642
  • DOI: 10.1111/eff.12307
  • Peer Reviewed
• [Journal Article] Techniques for the practical collection of environmental DNA: filter selection, preservation, and extraction (2016)
  • Author(s): Minamoto, T., Naka, T., Moji, K., and Maruyama, A.
  • Journal Title: Limnology Volume: 17 Pages: 23-32
  • Peer Reviewed
• [Journal Article] Environmental DNA as a ‘Snapshot’ of Fish Distribution: A Case Study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan (2016)
  • Author(s): S Yamamoto, K Minami, K Fukaya, K Takahashi, H Sawada, H Murakami, S Tsuji, H Hashizume, S Kubonaga, T Horiuchi, M Hongo, J Nishida, Y Okugawa, A Fujiwara, M Fukuda, S Hidaka, KW Suzuki, M Miya, H Araki, H Yamanaka, A Maruyama, K Miyashita, R Masuda, T Minamoto, M Kondoh
  • Journal Title: Plos One Volume: 10 Issue: 4 Pages: e0153291-e0153291
  • DOI: 10.1371/journal.pone.0153291
  • NAID: 120005733220
  • Peer Reviewed
• [Journal Article] Quick change in δ15N values of fish mucus confirmed in the field using a migratory goby (2015)
  • Author(s): Maruyama, A., Shimonaka, H., and Ito, T.
  • Journal Title: Ecology of Freshwater Fish Volume: 24 Pages: 162-164
  • Peer Reviewed
• [Presentation] 琵琶湖水系に陸封されたアユの安定同位体比からわかること～異なる時間スケールの食性を示す複数組織を組み合わせて～ (2018)
  • Author(s): 沢田隼，藤原壮平，遊磨正秀，丸山敦
  • Organizer: 日本生態学会
• [Presentation] 生きた魚からの粘液の反復採取と同位体比の変化速度 (2018)
  • Author(s): 植田誉規，久布真幸，重田環志，沢田 隼，米倉竜次，丸山敦
  • Organizer: 日本生態学会
• [Presentation] 体表粘液の安定同位体分析は淡水魚の短期間の食性を明らかにする (2018)
  • Author(s): 重田環志，米倉竜次，丸山敦
  • Organizer: 日本生態学会
• [Presentation] マラウィ湖産ヒレ食シクリッドの捕食行動の左右性 (2018)
  • Author(s): 竹内勇一，畑啓生，丸山敦，山田拓人，西川巧馬，Zatha Richard，Rusuwa Bosco，小田洋一
  • Organizer: 日本生態学会
• [Presentation] 農業水路網での魚類相調査における環境DNA分析の有用性 (2018)
  • Author(s): 丸山敦，麻田弥希，高田恭輔，渡邊和希，佐藤博俊，米倉竜次，山中裕樹
  • Organizer: 日本生態学会
• [Presentation] 魚食・鱗食・ヒレ食のシクリッド３種における口部形態の左右差の比較 (2018)
  • Author(s): 西川巧馬，丸山敦，畑啓生，Richard Zatha，Bosco Rusuwa，小田洋一，竹内勇一
  • Organizer: 日本生態学会
• [Presentation] マラウイ湖国立公園における森林資源の利用と保全 (2018)
  • Author(s): 林 珠乃, 遊磨正秀, 太田真人, Patrick Chinguwo, Bosco Rusuwa, Richard Zatha, Gibson Kamanje, 丸山敦
  • Organizer: 日本生態学会
• [Presentation] 多種共存するマラウイ湖シクリッド魚類群集の分布パターン (2018)
  • Author(s): 川上将樹，Richard Zatha，Bosco Rusuwa，丸山敦
  • Organizer: 日本生態学会
• [Presentation] 淡水魚の粘液、鰭、筋肉の置換速度の比較：応答の速い安定同位体分析法をめざして (2017)
  • Author(s): 重田環志，津馬汐莉，米倉竜次，丸山敦
  • Organizer: 日本生態学会
  • Place of Presentation: 東京
• [Presentation] 環境に関わらず重複するシクリッド魚類の同属種間のニッチ：胃内容の珪藻をマーカーにして (2017)
  • Author(s): 川上将樹，篠原耕平，大塚泰介，Bosco RUSUWA，丸山敦
  • Organizer: 日本生態学会
  • Place of Presentation: 東京
• [Presentation] 安定同位体比によるアユの産着卵の生活史型と産卵回数の推定 (2017)
  • Author(s): 沢田隼，重田環志，川上将樹，遊磨正秀，丸山敦
  • Organizer: 日本生態学会
  • Place of Presentation: 東京
• [Presentation] リンドウ科光合成植物の菌従属栄養性に関する研究 (2017)
  • Author(s): 鈴木貴子，丸山敦，遊川知久，大和政秀
  • Organizer: 日本生態学会
  • Place of Presentation: 東京
• [Presentation] 安定同位体比が示す琵琶湖水系に陸封されたアユの生活史変異とその産卵特性 (2016)
  • Author(s): 沢田隼，遊磨正秀，丸山敦
  • Organizer: 日本魚類学会
  • Place of Presentation: 岐阜
  • Year and Date: 2016-09-24
• [Presentation] マラウイ湖に生息するシクリッド魚類の摂食行動と食性分析から見えるニッチの柔軟性 (2016)
  • Author(s): 川上将樹，篠原耕平，大塚泰介，Bosco RUSUWA，遊磨正秀，丸山敦
  • Organizer: 日本魚類学会
  • Place of Presentation: 岐阜
  • Year and Date: 2016-09-24
• [Presentation] Change in stable isotope ratios in the epidermal mucus of slow-growing fish (2016)
  • Author(s): Maruyama A, Tanahashi E, Hirayama T, Yonakura R
  • Organizer: 日本生態学会
  • Place of Presentation: 仙台国際会議場
  • Year and Date: 2016-03-20
• [Presentation] アーバスキュラー菌根を形成するリンドウ科光合成植物における菌従属栄養性の探索 (2016)
  • Author(s): 鈴木貴子，丸山敦，遊川知久，大和政秀
  • Organizer: 菌根研究会
  • Place of Presentation: 千葉