Tranport and uptake of the liposomes composed of marine complex lipids

• Project/Area Number: 15K14798
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Hokkaido University
• Principal Investigator: TAKAHASHI KORETARO 北海道大学, 水産科学研究院, 特任教授 (90125328)
• Research Collaborator: LINDER Michel
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 水産複合脂質 / リポソーム / 小腸上皮細胞モデル / 腸管モデル / ホスファチジルコリン / ホスファチジルセリン / スルホキノボシルジアシルグリセロール / M細胞 / ファーゴサイトーシス / エンドサイトーシス / マルチラメラベシクル / マクロピノサイトーシス / 小腸上皮細胞 / 水産リン脂質

Outline of Final Research Achievements

This work was done to investigate the transport and uptake effects of the liposomes composed of marine complex lipids on two types of liposomes i.e. multi lamella vesicles (MLV) and small unilamellar vesicles (SUV) in small intestinal epithelial cell model. The results of experiments done with endocytosis inhibitors indicated some mechanisms of the individual complex lipid SUV and MLV liposomes. The results of atomic force microscopy (AFM) showed that the surface statuses of the cholesterol containing marine complex lipid liposomes were smooth, but it did not seem to affect the transport through the small intestinal epithelial cell model.

Research Products

• [Int'l Joint Research] ロレーヌ大学(フランス)
• [Int'l Joint Research] ロレーヌ大学(フランス)