Efficient search for cell cycle inhibitors from marine organisms by usinf Fucci cells
| Project/Area Number |
15K14799
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic life science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Matsunaga Shigeki 東京大学, 大学院農学生命科学研究科(農学部), 教授 (60183951)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 海洋生物 / 細胞周期 / Fucci2細胞 / 探索研究 / 抗がん剤 |
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| Outline of Final Research Achievements |
By using HeLa/Fucci2 cells, it is possible to observe the progression of cell cycle in live cells under microscope, enabling the differentiation of non-specific toxic compounds from specific cell-cycle inhibitors. We applied this technology to search for compounds that affect the cell-cycle of the HeLa/Fucci2 cells. From the brittle star collected at Ishigaki Island we isolated and characterized curacin E and from the marine sponge collected at Kurose, north to Hachijo Island, dragmacidins G and H, pyrazines substituted by two indoles and a guanidinoethylthio group.
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Report
(3 results)
Research Products
(4 results)
