| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Outline of Final Research Achievements |
By using HeLa/Fucci2 cells, it is possible to observe the progression of cell cycle in live cells under microscope, enabling the differentiation of non-specific toxic compounds from specific cell-cycle inhibitors. We applied this technology to search for compounds that affect the cell-cycle of the HeLa/Fucci2 cells. From the brittle star collected at Ishigaki Island we isolated and characterized curacin E and from the marine sponge collected at Kurose, north to Hachijo Island, dragmacidins G and H, pyrazines substituted by two indoles and a guanidinoethylthio group.
|
