| Project/Area Number |
15K14852
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
野地 智法 東北大学, 農学研究科, 准教授 (10708001)
秦 英司 国立研究開発法人農業・食品産業技術総合研究機構, その他, 研究員 (50355153)
林 智人 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, ユニット長 (90297630)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 黄色ブドウ球菌 / 乳房炎 / トランスポゾン / 病原微生物 / 形質転換 |
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| Outline of Final Research Achievements |
Staphylococcus aureus is the most important etiologic agent of bovine mastitis. To develop a novel prophylactic and/or treatment strategy, it is important to understand virulence factors and microbial physiology of S. aureus isolated from bovine mastitis. However, information about the mastitis-associated virulence factors is very limited. We therefore attempted to construct a transposon-mutagenized library using S. aureus SA5, which was isolated from bovine mastitis, to understand the bovine mastitis-related virulence factors in this study. As a result, although the frequency of transposition of the transposon into the SA5 genome was low, we confirmed that two candidate clones actually had a transposon insertion in their genome. Next, we focused on sortase because it presents several known virulence factors on the cell surface of this bacterium. We thus constructed an isogenic sortase-deficient mutant derived from SA5 using the homologous recombination technique.
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