Establishment of canine induced pluripotent cells using the CRISPR/Cas9 system
| Project/Area Number |
15K14865
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Nishimura Ryohei 東京大学, 大学院農学生命科学研究科(農学部), 教授 (80172708)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 犬 / iPS細胞 / 骨髄間葉系幹細胞 / 犬骨髄間葉系幹細胞 / 再生医療 / CRISPR/Cas9 |
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| Outline of Final Research Achievements |
Since it was difficult to generate canine iPS-like cells according to previous studies, we re-examined the stemness of the cells which we used for inducing the initializing factors. The cells used in this study were BM-PACs, which we established as novel mesenchymal stem cells, were more immature with less senescence than conventional canine bone marrow mesenchymal stem cells. In addition, we explored the promoting factor for proliferation ability of BM-PACs for inducing the initializing factors with retrovirus. We found that bFGF promoted the proliferation ability of BM-PAC with enhancing the immaturity. These results suggested that BM-PACs treated with bFGF are expected to be available for efficient induction of initializing factors to generate canine iPS-like cells.
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Report
(3 results)
Research Products
(1 results)
