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Time series analysis of unknown differentiation mechanisms and related genes for hair follicle generation

Research Project

Project/Area Number 15K14866
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Nishifuji Koji  東京農工大学, (連合)農学研究科(研究院), 准教授 (20365422)

Co-Investigator(Kenkyū-buntansha) 井手 香織  東京農工大学, (連合)農学研究科(研究院), 講師 (40550281)
角田 茂  東京大学, 医科学研究所, 准教授 (80345032)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords毛包 / ネスチン / 幹細胞 / バルジ領域 / 毛包幹細胞 / 上皮系幹細胞 / 神経堤幹細胞
Outline of Final Research Achievements

In this study, we investigated whether nestin-expressing neural stem cells and their descendants can be mixed in keratin 15-expressing hair follicle bulge stem cells. We also aimed to generate plasmid constructs to investigate the fate of epithelial-neural stem cells. In murine anagen and hair follicle bulge, keratin 15-expressing cells with features of nestin-expressing cells or their descendants could not be detected by immunofluorescence analysis. Meanwhile, we could successfully generated plusmid constructs that could express FlpERT2, which induces Flp-FRT site specific recombination under the presence of tamoxifen, by Cre-loxP recombination in keratin 15 and nestin co-expressing cells.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2016 Other

All Presentation (1 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Presentation] Progenitor cells expressing nestin, a neural crest stem cell marker differentiate into outer root sheath keratinocytes2016

    • Author(s)
      Saki Onishi, Fumika Yokoi, Kaori Ide, Manabu Ohyama, Koji Nishifuji
    • Organizer
      8th World Congress of Veterinary Dermatology
    • Place of Presentation
      Bordeaux, France
    • Year and Date
      2016-05-31
    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Int'l Joint Research
  • [Remarks]

    • URL

      http://kenkyu-web.tuat.ac.jp/Profiles/15/0001474/profile.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 東京農工大学研究者紹介 西藤公司

    • URL

      http://kenkyu-web.tuat.ac.jp/Profiles/15/0001474/profile.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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