Time series analysis of unknown differentiation mechanisms and related genes for hair follicle generation
| Project/Area Number |
15K14866
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Nishifuji Koji 東京農工大学, (連合)農学研究科(研究院), 准教授 (20365422)
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| Co-Investigator(Kenkyū-buntansha) |
井手 香織 東京農工大学, (連合)農学研究科(研究院), 講師 (40550281)
角田 茂 東京大学, 医科学研究所, 准教授 (80345032)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 毛包 / ネスチン / 幹細胞 / バルジ領域 / 毛包幹細胞 / 上皮系幹細胞 / 神経堤幹細胞 |
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| Outline of Final Research Achievements |
In this study, we investigated whether nestin-expressing neural stem cells and their descendants can be mixed in keratin 15-expressing hair follicle bulge stem cells. We also aimed to generate plasmid constructs to investigate the fate of epithelial-neural stem cells. In murine anagen and hair follicle bulge, keratin 15-expressing cells with features of nestin-expressing cells or their descendants could not be detected by immunofluorescence analysis. Meanwhile, we could successfully generated plusmid constructs that could express FlpERT2, which induces Flp-FRT site specific recombination under the presence of tamoxifen, by Cre-loxP recombination in keratin 15 and nestin co-expressing cells.
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Report
(4 results)
Research Products
(3 results)
