Establishment of method inducing DNA methylation by epigenome editing.

• Project/Area Number: 15K14924
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Kitasato University
• Principal Investigator: YAMAZAKI Taiga 北里大学, 北里大学メディカルセンター, 上級研究員 (90524231)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: DNAメチル化 / エピゲノム編集 / ペリセントロメア / セントロメア

Outline of Final Research Achievements

DNA methylation plays crucial roles in regulating gene expression, and aberrant DNA methylation leads developmental defects and diseases. Artificial control of DNA methylation in specific loci would be the valuable tool for both basic and applied research. In this research, we develop the method of epigenome editing especially in ‘Writing DNA methylation’ in mouse pericentromeres and centromeres.

Research Products

• [Journal Article] Expression of KK-LC-1, a cancer/testis antigen, at non-tumour sites of the stomach carrying a tumour (2018)
  • Author(s): Fukuyama Takashi、Futawatari Nobue、Yamamura Rui、Yamazaki Taiga、Ichiki Yoshinobu、Ema Akira、Ushiku Hideki、Nishi Yatsushi、Takahashi Yoshihito、Otsuka Toshikazu、Yamazaki Hitoshi、Koizumi Wasaburo、Yasumoto Kosei、Kobayashi Noritada
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 6131-6131
  • DOI: 10.1038/s41598-018-24514-9
  • Peer Reviewed / Open Access
• [Journal Article] Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase (2017)
  • Author(s): Yamazaki Taiga、Hatano Yu、Handa Tetsuya、Kato Sakiko、Hoida Kensuke、Yamamura Rui、Fukuyama Takashi、Uematsu Takayuki、Kobayashi Noritada、Kimura Hiroshi、Yamagata Kazuo
  • Journal Title: PLoS ONE Volume: 12 Issue: 5 Pages: 1-18
  • DOI: 10.1371/journal.pone.0177764
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Correlation Between Expression of the Cancer/Testis Antigen KK-LC-1 and Helicobacter pylori Infection in Gastric Cancer (2017)
  • Author(s): FUKUYAMA TAKASHI、FUTAWATARI NOBUE、ICHIKI YOSHINOBU、SHIDA AKIKO、YAMAZAKI TAIGA、NISHI YATSUSHI、NONOGUCHI HIROSHI、TAKAHASHI YOSHIHITO、YAMAZAKI HITOSHI、KOBAYASHI NORITADA
  • Journal Title: In Vivo Volume: 31 Issue: 3 Pages: 403-407
  • DOI: 10.21873/invivo.11073
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ゲノム編集技術を応用したペリセントロメアへの人為的・配列特異的DNAメチル化誘導 (2017)
  • Author(s): 山崎大賀、波多野 裕、半田哲也、加藤佐樹子、穂井田謙介、山村瑠衣、福山 隆、植松崇之、小林憲忠、木村 宏、山縣一夫
  • Organizer: 第11回日本エピジェネティクス研究会年会
• [Presentation] Targeted DNA methylation in pericentromere with genome editing based artificial DNA methyltransferase. (2016)
  • Author(s): Taiga Yamazaki, Kazuo Yamagata, Noritada Kobayashi
  • Organizer: International Symposium on Epigenome Dynamics and Regulation in Germ Cells
  • Place of Presentation: 京都府・京都大学百周年時計記念館
  • Year and Date: 2016-02-18
• [Presentation] ゲノム編集技術を応用したペリセントロメアへの人為的・配列特異的DNAメチル化誘導 (2016)
  • Author(s): 山崎 大賀、山縣 一夫、小林 憲忠
  • Organizer: 第1回日本ゲノム編集学会
  • Place of Presentation: 広島国際会議場（広島県・広島市）
• [Presentation] 細菌由来CpGメチル化酵素を利用したエピゲノム編集技術の開発 (2016)
  • Author(s): 山崎 大賀
  • Organizer: 第1回日本遺伝子細胞治療学会若手研究セミナー
  • Place of Presentation: 東京慈恵医科大学1号館講堂（東京都・港区）
• [Presentation] ゲノム編集技術を応用したペリセントロメアへの人為的・配列特異的DNAメチル化誘導 (2015)
  • Author(s): 山﨑大賀、山縣一夫、小林憲忠
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 兵庫県・神戸ポートアイランド
  • Year and Date: 2015-12-01