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Development of noninvasive and accurate theranostic nanodevices and analysis of their renal excretion

Research Project

Project/Area Number 15K14936
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Physical pharmacy
Research InstitutionShowa University (2017)
The University of Tokyo (2015-2016)

Principal Investigator

Kato Masaru  昭和大学, 薬学部, 教授 (30332943)

Co-Investigator(Kenkyū-buntansha) 三森 功士  九州大学, 大学病院, 教授 (50322748)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordscfDNA / 粒子 / 抽出 / 前処理 / 尿 / 診断 / DNA
Outline of Final Research Achievements

Cell free DNAs in urine and blood are attracting many attentions as biomarkers for diseases such as cancer. Hence, we developed a simple and efficient extraction method of cfDNA in body fluid using particles in this study. In order to extract negatively charged DNA, particles coated with various positive charges were prepared and quantity of DNA extraction was examined. We found that particles coated with polylysine or triamine or trimethylamine captured DNA efficiently and we succeeded in extraction of large amount of cfDNA from urine.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) Remarks (2 results)

  • [Int'l Joint Research] Ecole polytechnique federale de Lausanne(スイス)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Extraction of cell-free DNA from urine, using polylysine-coated silica particles.2017

    • Author(s)
      Takano S, Hu Q, Amamoto T, Refinetti P, Mimori K, Funatsu T, Kato M
    • Journal Title

      Anal Bioanal Chem.

      Volume: 409(16) Issue: 16 Pages: 4021-4025

    • DOI

      10.1007/s00216-017-0345-3

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 体液中cell-free DNA回収粒子の開発2017

    • Author(s)
      髙野 勝、村山周平、青木伊知男、船津高志、加藤 大
    • Organizer
      第 30回バイオメディカル分析科学シンポジウム(BMAS2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Poly-Lys修飾粒子を用いた尿中cell-freeDNAの回収2017

    • Author(s)
      高野 勝、船津 高志、加藤 大
    • Organizer
      新アミノ酸分析研究会第7回学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ナノ粒子を用いた生体液中DNA回収用デバイスの開発2016

    • Author(s)
      高野勝、村山周平、胡慶江、三森功士、青木伊知男、船津高志、加藤大
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 粒子を用いた生体液中DNA回収用デバイスの開発2016

    • Author(s)
      高野 勝、加藤 大
    • Organizer
      第20回日本がん分子標的治療学会学術集会
    • Place of Presentation
      別府国際コンベンションセンター(大分県 別府市)
    • Related Report
      2016 Research-status Report
  • [Remarks] 研究室のHP

    • URL

      http://www10.showa-u.ac.jp/~bachem/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 研究室のホームページ

    • URL

      http://park.itc.u-tokyo.ac.jp/CNBI/kato/index.html

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-12-27  

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