| Project/Area Number |
15K14936
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Showa University (2017)
The University of Tokyo (2015-2016) |
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Principal Investigator |
Kato Masaru 昭和大学, 薬学部, 教授 (30332943)
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| Co-Investigator(Kenkyū-buntansha) |
三森 功士 九州大学, 大学病院, 教授 (50322748)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | cfDNA / 粒子 / 抽出 / 前処理 / 尿 / 診断 / DNA |
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| Outline of Final Research Achievements |
Cell free DNAs in urine and blood are attracting many attentions as biomarkers for diseases such as cancer. Hence, we developed a simple and efficient extraction method of cfDNA in body fluid using particles in this study. In order to extract negatively charged DNA, particles coated with various positive charges were prepared and quantity of DNA extraction was examined. We found that particles coated with polylysine or triamine or trimethylamine captured DNA efficiently and we succeeded in extraction of large amount of cfDNA from urine.
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