| Project/Area Number |
15K14962
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pharmacology in pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
Akaike Akinori 京都大学, 薬学研究科, 客員教授 (80135558)
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| Co-Investigator(Kenkyū-buntansha) |
泉 安彦 京都大学, 薬学研究科, 助教 (60456837)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ヒトiPS由来細胞 / アルツハイマー病 / 薬効評価 |
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| Outline of Final Research Achievements |
We investigated whether the mechanism of Aβ-induced neurotoxicity accumulated in rat cultured neurons applied to human neurons and aimed to construct human iPS-based drug evaluation system for Alzheimer's disease. First, iPS cells were differentiated into neural stem cells (NSCs), and we obtained hiPS-differentiated NSCs which were not requiring feeder cells. Next, we examined that effect of neuron-astrocyte interaction on cytotoxicity using rat neuron monoculture and rat neuron-astrocyte mixed culture. It is suggested that the interaction between neuron and astrocyte reduce the susceptibility to Aβ-induced neurotoxicity.
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