Functional analysis of "angio-lymphatic vessels" in the neoplastic lesions - its histopathological significance

• Project/Area Number: 15K15011
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Hirosaki University
• Principal Investigator: Kijima Hiroshi 弘前大学, 医学研究科, 教授 (90204859)
• Co-Investigator(Kenkyū-buntansha): 下田 浩 弘前大学, 医学研究科, 教授 (20274748)
• Co-Investigator(Renkei-kenkyūsha): MOROHASHI SATOKO 弘前大学, 大学院医学研究科, 助教 (90569592) ; WU YUNYAN 弘前大学, 大学院医学研究科, 助教 (40636586)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 血管新生 / 腫瘍細胞 / 浸潤性増殖 / 血行性転移 / リンパ行性転移

Outline of Final Research Achievements

Angiogenesis and stromal invasion play important roles in tumor growth/extension. We have identified angio-lymphatic vessels positive for both vascular marker (factor VIII) and lymphatic marker (D2-40). We examined histopathological characteristics of the aigio-lymphatic vessels in the tumors. The angio-lymphatic vessels are found not only tumor tissues, but also their surrounding tissues, and thought to play a significant role in early tumor invasion and neoangiogenesis.

Research Products

• [Journal Article] Expression of cancer-associated fibroblast markers in advanced colorectal cancer (2018)
  • Author(s): Nishishita Rie、Morohashi Satoko、Seino Hiroko、Wu Yunyan、Yoshizawa Tadashi、Haga Toshihiro、Saito Kensuke、Hakamada Kenichi、Fukuda Shinsaku、Kijima Hiroshi
  • Journal Title: Oncol Lett. Volume: 15 Pages: 6195-6202
  • DOI: 10.3892/ol.2018.8097
  • Peer Reviewed / Open Access
• [Journal Article] Epithelial-mesenchymal Transition (EMT) is Correlated with Patient's Prognosis of Lung Squamous Cell Carcinoma. (2018)
  • Author(s): Aruga N, Kijima H, Masuda R, Onozawa H, Yoshizawa T, Tanaka M, Inokuchi S, Iwazaki M.
  • Journal Title: Tokai J Exp Clin Med. Volume: 43 Pages: 5-13
  • Peer Reviewed
• [Journal Article] Prognostic Impact of Extracapsular Lymph Node Invasion and Myofibroblastic Activity in Extrahepatic Bile Duct Cancer (2017)
  • Author(s): Yoshizawa Tadashi、Ishido Keinosuke、Saito Kensuke、Haga Toshihiro、Seino Hiroko、Wu Yunyan、Morohashi Satoko、Hakamada Kenichi、Kijima Hiroshi
  • Journal Title: Clin Med Insights Pathol. Volume: 10 Pages: 1-7
  • DOI: 10.1177/1179555717729652
  • NAID: 120006583346
  • Peer Reviewed / Open Access
• [Journal Article] <b>CCL5 is induced by TLR 3 signaling in HuCCT1 human biliary epithelial cells: possible involvement in the pathogenesis of biliary atr</b><b>esia </b> (2017)
  • Author(s): Shimada T, Imaizumi T, Shirai K, Tatsuta T, Hayakari R, Yoshida H, Matsumiya T, Kimura T, Kijima K, Mizukami H, Hakamada K.
  • Journal Title: Biomedical Research Volume: 38 Issue: 5 Pages: 269-276
  • DOI: 10.2220/biomedres.38.269
  • NAID: 130006179806
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access
• [Journal Article] DEC1 and DEC2 Regulate Apoptosis of Human Prostate Adenocarcinoma LNCap Cells. (2017)
  • Author(s): Liu Q, Wu Y, Seino H, Yoshizawa T, Haga T, Morohashi S, Kijima H.
  • Journal Title: Biomed Res J. Volume: 1 Pages: 6-11
  • Peer Reviewed / Open Access
• [Journal Article] Lymphatic invasion is a significant indicator of poor patient prognosis in lung squamous cell carcinoma. (2017)
  • Author(s): Masuda R, Kijima H, Nito M, Wada A, Matsuzaki T, Ikoma Y, Nakazato K, Masuda D, Tanaka M, Kobayashi H, Inokuchi S, Iwazaki M.
  • Journal Title: Mol Med Rep. Volume: 15 Issue: 4 Pages: 2067-2073
  • DOI: 10.3892/mmr.2017.6256
  • Peer Reviewed / Open Access
• [Journal Article] Prognostic Impact of CD163+ Macrophages in Tumor Stroma and CD8+ T-Cells in Cancer Cell Nests in Invasive Extrahepatic Bile Duct Cancer. (2017)
  • Author(s): Miura T, Yoshizawa T, Hirai H, Seino H, Morohashi S, Wu Y, Wakiya T, Kimura N, Kudo D, Ishido K, Toyoki Y, Kijima H, Hakamada K.
  • Journal Title: Anticancer Res. Volume: 37 Pages: 183-190
  • Peer Reviewed / Open Access
• [Journal Article] Basic helix-loop-helix transcription factor DEC2 functions as an anti-apoptotic factor during paclitaxel-induced apoptosis in human prostate cancer cells. (2016)
  • Author(s): Liu Q, Wu Y, Yoshizawa T, Yan X, Morohashi S, Seino H, Kato Y, Kijima H.
  • Journal Title: Int J Mol Med Volume: 38 Issue: 6 Pages: 1727-1733
  • DOI: 10.3892/ijmm.2016.2798
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Myofibroblast distribution is associated with invasive growth types of colorectal cancer. (2016)
  • Author(s): Takatsuna M, Morohashi S, Yoshizawa T, Hirai H, Haga T, Ota R, Saito K, Wu Y, Seino H, Aoyagi Y, Terai S, Kijima H.
  • Journal Title: Oncol Rep Volume: 36 Issue: 6 Pages: 3154-3160
  • DOI: 10.3892/or.2016.5202
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Clinicopathological significance of gastric poorly differentiated medullary carcinoma. (2016)
  • Author(s): Hirai H, Yoshizawa T, Morohashi S, Haga T, Wu Y, Ota R, Takatsuna M, Akasaka H, Hakamada K, Kijima H.
  • Journal Title: Biomed Res. Volume: 37 Pages: 77-84
  • NAID: 130005147429
  • Open Access / Acknowledgement Compliant
• [Journal Article] Clinicopathological significance of vascular endothelial growth factor, thymidine phosphorylase and microvessel density in colorectal cancer. (2016)
  • Author(s): 5.Kimura Y, Morohashi S, Yoshizawa T, Suzuki T, Morohashi H, Sakamoto Y, Koyama M, Murata A, Kijima H, Hakamada K.
  • Journal Title: Mol Med Rep. Volume: 13 Issue: 2 Pages: 1551-1557
  • DOI: 10.3892/mmr.2015.4687
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Imaging hamster model of bile duct cancer in vivo using fluorescent L-glucose derivatives. (2016)
  • Author(s): 3.Yokoyama H, Sasaki A, Yoshizawa T, Kijima H, Hakamada K, Yamada K.
  • Journal Title: Hum Cell. Volume: Epub ahead of print
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expression of podoplanin and vimentin is correlated with prognosis in esophageal squamous cell carcinoma. (2015)
  • Author(s): 7.Tanaka M, Kijima H, Shimada H, Makuuchi H, Ozawa S, Inokuchi S.
  • Journal Title: Mol Med Rep. Volume: 12 Issue: 3 Pages: 4029-4036
  • DOI: 10.3892/mmr.2015.3966
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] <b>Podoplanin expression is correlated with the prognosis of lung squamous cell car</b><b>cinoma </b> (2015)
  • Author(s): 4.Ikoma Y, Kijima H, Masuda R, Tanaka M, Inokuchi S, Iwazaki M.
  • Journal Title: Biomedical Research Volume: 36 Issue: 6 Pages: 393-402
  • DOI: 10.2220/biomedres.36.393
  • NAID: 130005116834
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] The bHLH transcription factor DEC plays an important role in epithelial-mesenchymal transition (EMT). (2016)
  • Author(s): Hiroshi Kijima, Yunyan Wu, Tadashi Yoshizawa, Hiroko Seino, Shintaro Goto, Toshihiro Haga, Satoko Morohashi.
  • Organizer: 21st World Congress on Advances in Oncology and 19th International Symposium on Molecular Medicine.
  • Place of Presentation: Athens, (Greece)
  • Year and Date: 2016-10-06
  • Int'l Joint Research / Invited
• [Presentation] BHLH transcription factor DEC1 promotes hypoxia-induced EMT of HepG2 cells. (2016)
  • Author(s): Keishu Murakami, Yunyan Wu, Yuka Aoki, Hiroko Seino, Satoko Morohashi, Tadaatsu Imaizumi, Hiroshi Kijima.
  • Organizer: 21st World Congress on Advances in Oncology and 19th International Symposium on Molecular Medicine.
  • Place of Presentation: Athens, (Greece)
  • Year and Date: 2016-10-06
  • Int'l Joint Research
• [Book] 腫瘍病理鑑別診断アトラス｢胆道癌･膵癌」 (2015)
  • Author(s): 鬼島 宏・福嶋敬宜（編著）
  • Total Pages: 331
  • Publisher: 文光堂
• [Remarks] 弘前大学大学院医学研究科病理生命科学講座 ホームページ
  • URL: http://www.med.hirosaki-u.ac.jp/~patho2/