| Project/Area Number |
15K15021
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Kitasato University |
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Principal Investigator |
Kadoya Yuichi 北里大学, 医療衛生学部, 教授 (10185887)
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| Co-Investigator(Kenkyū-buntansha) |
二木 杉子 大阪医科大学, 医学部, 助教 (00403014)
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| Research Collaborator |
Sekiguchi Kiyotoshi 大阪大学, 蛋白質研究所, 教授
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 基底膜 / ライブイメージング / ナイドゲン(nidogen) / 顎下腺 / 分枝形態形成 / EGFP / ナイドジェン / MMP阻害剤 |
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| Outline of Final Research Achievements |
We have recently generated recombinant human nidogen-1 whose C-terminus is fused to enhanced green fluorescent protein (hNid1-EGFP). Because nidogen-1 binds to several other major BM proteins, an attempt was made to label BM of living tissues with exogenous hNid1-EGFP and to reveal BM dynamics. Developing submandibular gland (SMG) of mouse was chosen because of its rapid expansion of BM as a result of epithelial branching. Embryonic day-13 SMG was organ cultured in the glass-bottom-dish with a medium containing hNid1-EGFP. After 15min of incubation, confocal microscopy revealed linear deposition of hNid1-EGFP sin the epithelial BMs.
We conclude that hNid1-EGFP is a unique and useful tool to study BM dynamics in vitro.
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