| Project/Area Number |
15K15093
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Pathological medical chemistry
|
|---|
| Research Institution | National Cancer Center Japan |
|---|
Principal Investigator |
Arakawa Hirofumi 国立研究開発法人国立がん研究センター, 研究所, 分野長 (70313088)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | リソソーム / ミトコンドリア / タンパク質分解 / 品質管理 / 活性酸素種 / 酸化ストレス / 酸化タンパク質 / p53 |
|---|
| Outline of Final Research Achievements |
In order to evaluate the existence of the intramitochondrial lysosome, we performed electron microscopic analysis using the various organs and/or tissues of Mieap-wild and knockout mice. As the result, we did not morphologically observe any lysosome-like structures within the mitochondria. However, in the live-imaging experiment with various cell lines, we succeeded to visualize the process of the Mieap-induced accumulation of lysosome-like organelles within mitochondria. These results suggest that Mieap-regulated mitochondrial quality control is critically different from canonical lysosomal function.
|
