Analysis of human intestinal microbiota
| Project/Area Number |
15K15152
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Immunology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Shota 大阪大学, 微生物病研究所, 特任准教授 (90432434)
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| Research Collaborator |
MAEDA Yuichi
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 関節リウマチ / 腸内細菌 / 免疫 / Th17 |
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| Outline of Final Research Achievements |
Composition of intestinal microbiota has been shown to be altered in several disorders including rheumatoid arthritis (RA). However, it remained unknown whether the altered composition of microbiota is a result or cause of RA. We found that a subpopulation of RA patients harbored Prevotella-dominated intestinal microbiota. We analyzed the altered composition of microbiota dominated by Prevotella contributes to the pathogenesis of arthritis in arthritis-prone SKG mice, which did not develop arthritis in the absence of microbiota. Germ-free SKG mice were colonized with human microbiota (RA-type and healthy control (HC)-type microbiota). RA-SKG mice developed severe arthritis compared to SKG mice harboring HC microbiota. The number of Th17 cells in regional lymph nodes and the colon was increased in RA-SKG mice. Thus altered composition of microbiota contributes to the pathogenesis of arthritis.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] E-NPP3 controls plasmacytoid dendritic cell numbers in the small intestine.2017
Author(s)
Furuta, Y., Tsai, S-H., Kinoshita, M., Fujimoto, K., Okumura, R., Umemoto, E., Kurashima, Y, Kiyono, H., Kayama, H., Takeda, K.
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Journal Title
Mucosal Immunology
Volume: 12
Issue: 2
Pages: e0172509-e0172509
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Regulation of intestinal homeostasis by theulcerative colitis-associated gene RNF186.2017
Author(s)
Fujimoto K, Kinoshita M, Tanaka H, Okuzaki D, Shimada Y, Kayama H, Okumura R, Furuta Y, Narazaki M, Tamura A, Hatakeyama S, Ikawa M, Tsuchiya K, Watanabe M, Kumanogoh A, Tsukita S, Takeda K
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Journal Title
Mucosal Immunol.
Volume: 10
Issue: 2
Pages: 446-459
DOI
Related Report
Peer Reviewed
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[Journal Article] Dysbiosis contributes to arthritis development via activation of autoreactive T cells in the intestine.2016
Author(s)
Maeda Y, Kurakawa T, Umemoto E, Motooka D, Ito Y, Gotoh K, Hirota K, Matsushita M, Furuta Y, Narazaki M, Sakaguchi N, Kayama H, Nakamura S, Iida T, Saeki Y, Kumanogoh A, Sakaguchi S, and Takeda K
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Journal Title
Arthritis Rheum
Volume: 68
Issue: 11
Pages: 2646-2661
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Lypd8 promotes the segregation of flagellated microbiota and colonic epithelia.2016
Author(s)
Okumura R, Kurakawa T, Nakano T, Kayama H, Kinoshita M, Motoka D, Gotoh K, Kimura T, Kamiyama N, Kusu T, Ueda Y, Wu H, Iijima H, Barman S, Osawwa H, Matsuno H, Nishimura J, Ohba Y, Nakamura S, Iida T, Yamamoto M, Umemoto E, Sano K, and Takeda K
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Journal Title
Nature
Volume: 532
Issue: 7597
Pages: 117-121
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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