• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of human intestinal microbiota

Research Project

Project/Area Number 15K15152
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Immunology
Research InstitutionOsaka University

Principal Investigator

Takeda Kiyoshi  大阪大学, 医学系研究科, 教授 (20309446)

Co-Investigator(Renkei-kenkyūsha) NAKAMURA Shota  大阪大学, 微生物病研究所, 特任准教授 (90432434)
Research Collaborator MAEDA Yuichi  
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords関節リウマチ / 腸内細菌 / 免疫 / Th17
Outline of Final Research Achievements

Composition of intestinal microbiota has been shown to be altered in several disorders including rheumatoid arthritis (RA). However, it remained unknown whether the altered composition of microbiota is a result or cause of RA. We found that a subpopulation of RA patients harbored Prevotella-dominated intestinal microbiota. We analyzed the altered composition of microbiota dominated by Prevotella contributes to the pathogenesis of arthritis in arthritis-prone SKG mice, which did not develop arthritis in the absence of microbiota. Germ-free SKG mice were colonized with human microbiota (RA-type and healthy control (HC)-type microbiota). RA-SKG mice developed severe arthritis compared to SKG mice harboring HC microbiota. The number of Th17 cells in regional lymph nodes and the colon was increased in RA-SKG mice. Thus altered composition of microbiota contributes to the pathogenesis of arthritis.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (23 results)

All 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 5 results) Presentation (14 results) (of which Int'l Joint Research: 7 results,  Invited: 14 results)

  • [Int'l Joint Research] THSTI(インド)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Scrips Institute(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] E-NPP3 controls plasmacytoid dendritic cell numbers in the small intestine.2017

    • Author(s)
      Furuta, Y., Tsai, S-H., Kinoshita, M., Fujimoto, K., Okumura, R., Umemoto, E., Kurashima, Y, Kiyono, H., Kayama, H., Takeda, K.
    • Journal Title

      Mucosal Immunology

      Volume: 12 Issue: 2 Pages: e0172509-e0172509

    • DOI

      10.1371/journal.pone.0172509

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection.2017

    • Author(s)
      Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, Kumanogoh A, Murakami M, Ikawa M and Takeda K
    • Journal Title

      J. Exp. Med.

      Volume: 印刷中 Issue: 5 Pages: 1313-1331

    • DOI

      10.1084/jem.20161076

    • NAID

      120006957435

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Regulation of intestinal homeostasis by theulcerative colitis-associated gene RNF186.2017

    • Author(s)
      Fujimoto K, Kinoshita M, Tanaka H, Okuzaki D, Shimada Y, Kayama H, Okumura R, Furuta Y, Narazaki M, Tamura A, Hatakeyama S, Ikawa M, Tsuchiya K, Watanabe M, Kumanogoh A, Tsukita S, Takeda K
    • Journal Title

      Mucosal Immunol.

      Volume: 10 Issue: 2 Pages: 446-459

    • DOI

      10.1038/mi.2016.58

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a human intestinal myeloid cell subset that regulates the gut homeostasis.2016

    • Author(s)
      Barman S, Kayama H, Okuzaki D, Ogino T, Osawa H, Matsuno H, Mizushima T, Mori M, Nishimura J and Takeda K
    • Journal Title

      Int Immunol

      Volume: 28 Issue: 11 Pages: 533-545

    • DOI

      10.1093/intimm/dxw034

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dysbiosis contributes to arthritis development via activation of autoreactive T cells in the intestine.2016

    • Author(s)
      Maeda Y, Kurakawa T, Umemoto E, Motooka D, Ito Y, Gotoh K, Hirota K, Matsushita M, Furuta Y, Narazaki M, Sakaguchi N, Kayama H, Nakamura S, Iida T, Saeki Y, Kumanogoh A, Sakaguchi S, and Takeda K
    • Journal Title

      Arthritis Rheum

      Volume: 68 Issue: 11 Pages: 2646-2661

    • DOI

      10.1002/art.39783

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] IL-10-producing lung interstitial macrophages prevent neutrophilic asthma.2016

    • Author(s)
      4Kawano H, Kayama H, Nakama T, Hashimoto T, Umemoto E, and Takeda K
    • Journal Title

      International Immunology

      Volume: in press

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Lypd8 promotes the segregation of flagellated microbiota and colonic epithelia.2016

    • Author(s)
      Okumura R, Kurakawa T, Nakano T, Kayama H, Kinoshita M, Motoka D, Gotoh K, Kimura T, Kamiyama N, Kusu T, Ueda Y, Wu H, Iijima H, Barman S, Osawwa H, Matsuno H, Nishimura J, Ohba Y, Nakamura S, Iida T, Yamamoto M, Umemoto E, Sano K, and Takeda K
    • Journal Title

      Nature

      Volume: 532 Issue: 7597 Pages: 117-121

    • DOI

      10.1038/nature17406

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 腸内細菌叢と腸管炎症制御2017

    • Author(s)
      竹田潔
    • Organizer
      第28回日本臨床微生物学会総会・学術集会
    • Place of Presentation
      長崎
    • Year and Date
      2017-01-20
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Regulation of intestinal homeostasis by epithelial barriers.2016

    • Author(s)
      竹田潔
    • Organizer
      International Symposium on Advanced Immunology
    • Place of Presentation
      大阪
    • Year and Date
      2016-11-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Segregation of intestinal bacteria and epithelia by Lypd8.2016

    • Author(s)
      竹田潔
    • Organizer
      The 15th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      兵庫
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 腸管上皮バリアからみた腸管恒常性維持機構2016

    • Author(s)
      竹田潔
    • Organizer
      第53回日本消化器免疫学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Regulation of intestinal homeostasis through segregation of microbiota and epithelia.2016

    • Author(s)
      竹田潔
    • Organizer
      The 4th Annual Meeting of Asian Organization for Chron’s & Colitis
    • Place of Presentation
      京都
    • Year and Date
      2016-07-08
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 腸管腔内アデノシン3リン酸(ATP)による免疫応答とその制御2016

    • Author(s)
      竹田潔, Tsai SH
    • Organizer
      第27回日本生体防御学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 腸管炎症の制御機構2016

    • Author(s)
      竹田潔
    • Organizer
      第37回日本炎症・再生医学会
    • Place of Presentation
      京都
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Altered composition of microbiota contributes to arthritis development2016

    • Author(s)
      竹田潔
    • Organizer
      The 1st International Symposium of Center for Integrative Rheumatoid Transcriptomics and Dynamics
    • Place of Presentation
      Seoul、韓国
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 腸管免疫2016

    • Author(s)
      竹田潔
    • Organizer
      第61回日本透析医学会学術集会総会
    • Place of Presentation
      大阪
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 腸内細菌叢と疾患のABC2016

    • Author(s)
      竹田潔
    • Organizer
      第60回日本リウマチ学会総会学術集会
    • Place of Presentation
      横浜
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Regulation of ATP-dependent immune responses2015

    • Author(s)
      Kiyoshi Takeda
    • Organizer
      The 43rd JSI Annual Meeting
    • Place of Presentation
      札幌
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] The mechanism for segregation of intestinal bacteria and epithelia in the colon.2015

    • Author(s)
      Kiyoshi Takeda
    • Organizer
      The 43rd JSI Annual Meeting
    • Place of Presentation
      札幌
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Maintenance of gut homeostasis by intestinal epithelial cells2015

    • Author(s)
      Kiyoshi Takeda
    • Organizer
      The 3rd Symposium of International Immunological Memory and Vaccine Forum
    • Place of Presentation
      Berlin, Germany
    • Year and Date
      2015-10-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 炎症性腸疾患の発症機構の解明をめざして2015

    • Author(s)
      竹田潔
    • Organizer
      第43回日本臨床免疫学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-10-22
    • Related Report
      2015 Research-status Report
    • Invited

URL: 

Published: 2015-04-16   Modified: 2022-02-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi