Fundamental Study on Preparations for Therapeutic Antibodies with Less Productions of Anti-Drug Antibodies

Research Project

Project/Area Number	15K15184
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Applied pharmacology
Research Institution	Kyushu University
Principal Investigator	Ueda Tadashi 九州大学, 薬学研究院, 教授 (90184928)
Co-Investigator(Kenkyū-buntansha)	日下部宜宏九州大学, 農学研究院, 教授 (30253595) 宗孝紀東北大学, 医学系研究科, 准教授 (60294964) 阿部義人九州大学, 薬学研究院, 准教授 (60315091) 白石充典九州大学, 薬学研究院, 助教 (00380527)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	antibody / stability / immunolgy / Anti-Drug Antibodies / Derp2 / Fab / 安定化 / 免疫学 / 蛋白質 / 抗体 / 安定性
Outline of Final Research Achievements	Anti-Drug Antibodies (ADAs) are often found in patients whom therapeutic antibodies are administrated. ADAs will lead the attenuation of medical effects of therapeutic antibodies. From the standpoints of reduction in medical costs, we should develop the method to depress ADAs as early as possible. In order to depress ADAs in general, we focus on the constant region of humanized Fab, which are common in therapeutic antibodies. In this study, we prepared the humanized Fab with different conformational stability by the modification of the constant regions and examined the immunogenicities of these human Fab with different conformational stabilities.