| Project/Area Number |
15K15197
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大山 陽子 鹿児島大学, 附属病院, 特任助教 (20583470)
竹之内 和則 鹿児島大学, 附属病院, 医員 (30646758)
清水 利昭 鹿児島大学, 医歯学域医学系, 助教 (50468055)
山口 宗一 鹿児島大学, 医歯学域医学系, 准教授 (20325814)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 血管新生 / 血球貪食 / 鉄 / 免疫 / リンパ管新生 / リンパ節 / B細胞 / VEGF / 赤血球 / 免疫寛容 / 脾臓 / erythrocyte / eryptosis / programmed cell deat / VEGF-A / lymph node / phagocytosis / iron metabolism |
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| Outline of Final Research Achievements |
Aim: Fine tuning the immune response through hemophagocytosis:Materials and methods: The CD19Cre/hVEGF-Afl mice were employed for this study. H&E staining and immunohistochemical staining were performed to identify hemophagocytosis in LNs. Prussian blue staining was done for quantification of iron deposition in tissues. The number of CD8+ T cells and programmed cell death 1 (PD-1) positive cells in LNs were analyzed by flow cytometry. Hepcidin expression in the liver was analyzed by RT-PCR. Result: The active hemophagocytosis in LNs of CD19Cre/hVEGF-Afl mice were observed together with decreasing the number of CD8+ T cells and increasing PD-1 expression in CD8+ T cells. Also CD19Cre/hVEGF-Afl mice represented the phenotype corresponding with iron deficiency anemia. Conclusion: B-cell derived VEGF-A executes tuning of immune response by decreasing the number of CD8+ T cells, increasing PD-1 expression in CD8+ T cells and hemophagocytosis.
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