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Fine tuning the immune response through physiological hemophagocytosis

Research Project

Project/Area Number 15K15197
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionKagoshima University

Principal Investigator

Hashiguchi Teruto  鹿児島大学, 医歯学域医学系, 教授 (70250917)

Co-Investigator(Kenkyū-buntansha) 大山 陽子  鹿児島大学, 附属病院, 特任助教 (20583470)
竹之内 和則  鹿児島大学, 附属病院, 医員 (30646758)
清水 利昭  鹿児島大学, 医歯学域医学系, 助教 (50468055)
山口 宗一  鹿児島大学, 医歯学域医学系, 准教授 (20325814)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords血管新生 / 血球貪食 / 鉄 / 免疫 / リンパ管新生 / リンパ節 / B細胞 / VEGF / 赤血球 / 免疫寛容 / 脾臓 / erythrocyte / eryptosis / programmed cell deat / VEGF-A / lymph node / phagocytosis / iron metabolism
Outline of Final Research Achievements

Aim: Fine tuning the immune response through hemophagocytosis:Materials and methods: The CD19Cre/hVEGF-Afl mice were employed for this study. H&E staining and immunohistochemical staining were performed to identify hemophagocytosis in LNs. Prussian blue staining was done for quantification of iron deposition in tissues. The number of CD8+ T cells and programmed cell death 1 (PD-1) positive cells in LNs were analyzed by flow cytometry. Hepcidin expression in the liver was analyzed by RT-PCR. Result: The active hemophagocytosis in LNs of CD19Cre/hVEGF-Afl mice were observed together with decreasing the number of CD8+ T cells and increasing PD-1 expression in CD8+ T cells. Also CD19Cre/hVEGF-Afl mice represented the phenotype corresponding with iron deficiency anemia. Conclusion: B-cell derived VEGF-A executes tuning of immune response by decreasing the number of CD8+ T cells, increasing PD-1 expression in CD8+ T cells and hemophagocytosis.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Low grade inflammation inhibits VEGF induced HUVECs migration in p53 dependent manner.2017

    • Author(s)
      Panta S, Yamakuchi M, Shimizu T, Takenouchi K, Oyama Y, Koriyama T, Kojo T, Hashiguchi T.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 483 Issue: 2 Pages: 803-809

    • DOI

      10.1016/j.bbrc.2016.12.096

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Switch in the Dynamics of Intra-Platelet VEGF-A from Cancer to the Later Phase of Liver Regeneration after Partial Hepatectomy in Humans2016

    • Author(s)
      Aryal B, Shimizu T, Kadono J, Furoi A, Komokata T, Inoue M, Ikeda S, Fukukura Y, Nakamura M, Yamakuchi M, Hashiguchi T, Imoto Y
    • Journal Title

      PLoS One

      Volume: 11(3) Issue: 3 Pages: e0150446-e0150446

    • DOI

      10.1371/journal.pone.0150446

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Platelet derived growth factors predict HCC recurrence after partial hepatectomy in humans2017

    • Author(s)
      Bibek Aryal、清水利昭、門野 潤、風呂井 彰、山口宗一、菰方輝夫、橋口照人、井本 浩
    • Organizer
      第62回日本臨床検査医学会 九州地方会 第28回日本臨床化学会 九州支部総会
    • Place of Presentation
      久留米大学病院 筑水会館(福岡県久留米市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] CalDAG-GEFI分子異常による血小板機能異常症2017

    • Author(s)
      古城 剛、山口宗一、吉村明子、竹之内和則、伊藤隆史、清水利昭、大山陽子、郡山豊泰、波野史典、Sushil Panta 、松下昌風、丸山征郎、髙嶋 博、橋口照人
    • Organizer
      第62回日本臨床検査医学会 九州地方会 第28回日本臨床化学会 九州支部総会
    • Place of Presentation
      久留米大学病院 筑水会館(福岡県久留米市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Tracking the signaling pathway for the anti-angiogenic activity of proinflammatory factor TNF alpha2017

    • Author(s)
      Sushil Panta、山口宗一、清水利昭、竹之内和則、大山陽子、郡山豊泰、古城 剛、橋口照人
    • Organizer
      第62回日本臨床検査医学会 九州地方会 第28回日本臨床化学会 九州支部総会
    • Place of Presentation
      久留米大学病院 筑水会館(福岡県久留米市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Co-operation of TNFalpha and VEGF to regulate PAI-1 through synergistically upregulated p532016

    • Author(s)
      Sushil Panta, Munekazu Yamakuchi, Toshiaki Shimizu, Kazunori Takenouchi, Yoko Oyama, Teruto Hashiguchi
    • Organizer
      XXlllrd International Congress on Fibrinolysis & Proteolysis and XVlth International Workshop on Molecular and Cellular Biology of Plasminogen Activation
    • Place of Presentation
      Nippondaira Hotel, Nihondaira, Shizuoka, Japan(静岡県静岡市)
    • Year and Date
      2016-10-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Recent Topic & Future Development of coagulation therapy2016

    • Author(s)
      Teruto Hashiguchi
    • Organizer
      9th Congress of the Asian-Pacific Society on Thrombosis and Hemostasis
    • Place of Presentation
      Taipei International Convention Center, Taipei City, Taiwan(台湾台北市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] L.pneumophila感染マクロファージ系細胞におけるmiRNAの役割2016

    • Author(s)
      山口宗一、郡山豊泰、古城 剛、竹之内和則、清水利昭、大山陽子、松下昌風、橋口照人
    • Organizer
      第38回日本血栓止血学会学術集会
    • Place of Presentation
      奈良春日野国際フォーラム~I・RA・KA~(奈良県奈良市)
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] CalDAG-GEFI分子異常により血小板機能異常を呈した一症例2016

    • Author(s)
      古城 剛、山口宗一、吉村明子、竹之内和則、伊藤隆史、清水利昭、大山陽子、郡山豊泰、Sushil Panta 、松下昌風、丸山征郎、髙嶋 博、橋口照人
    • Organizer
      第38回日本血栓止血学会学術集会
    • Place of Presentation
      奈良春日野国際フォーラム~I・RA・KA~(奈良県奈良市)
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] A bidirectional role of platelet sequestered vascular endothelial growth factor-A in cancer and liver regeneration.2015

    • Author(s)
      Bibek Aryal, Toshiaki Shimizu, Jun Kadono, Akira Furoi, Maki Inoue, Teruo Komokata, Munekazu Yamakuchi, Teruto Hashiguchi, Yutaka Imoto.
    • Organizer
      第62回日本臨床検査医学会学術集会
    • Place of Presentation
      岐阜県岐阜市長良川国際会議場
    • Year and Date
      2015-11-19
    • Related Report
      2015 Research-status Report
  • [Presentation] Platelet Mediated Angiogenesis During Liver regeneration After Partial Hepatectomy In Humans.2015

    • Author(s)
      Bibek Aryal, Toshiaki Shimizu, Jun Kadono, Akira Furoi, Maki Inoue, Teruo Komokata, Munekazu Yamakuchi, Teruto Hashiguchi, Yutaka Imoto.
    • Organizer
      第27回日本肝胆膵外科学会学術集会
    • Place of Presentation
      東京都港区ホテル グランパシフィック LE DAIBA
    • Year and Date
      2015-06-11
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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