Explication of cholesterol efflux mechanism from small intestine
| Project/Area Number |
15K15349
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Metabolomics
|
|---|
| Research Institution | Nagoya City University |
|---|
Principal Investigator |
TSUJITA MAKI 名古屋市立大学, 大学院医学研究科, 講師 (10253262)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
鵜川 眞也 名古屋市立大学, 大学院医学研究科, 教授 (20326135)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | SR-BI flox / CAG cre / Villin cre / mouse apoA-I / mouse HDL / mouse LDL / TICE / HDL / CAG-Cre / Vil-Cre / Alb-Cre / マウス / 小腸 / mouse / cholesterol / LCAT / ABCA1 / SR-BI / Probucol |
|---|
| Outline of Final Research Achievements |
The TICE is alternative pathway for reverse cholesterol transport. Acceleration of TICE pathway may become one of the new strategies without causing cholesterol polypus leading cholecystolithiasis, or gallbladder inflammation. We aim to evaluate SR-BI function on TICE which SR-BI receptor also localized at basolateral membrane in intestinal absorptive cell. In the project, SR-BI flox and CAG-Cre mice were obtained. The SR-BI fx/fx-CAG+ mice was created. The plasma analysis indicated accumulation of large HDL. This result proved the quality of the SR-BI flox mice and current propagation system. Vil-Cre mice and Alb-Cre mice have arrived from the collaborator to create intestinal- and liver-specific SR-BI KO mic for the future study. WT mice plasma was harvested and the isolated mouse LDL, HDL, and mouse apoA-I were shipped to the company and the rat monoclonal antibodies were successfully ready. They will be also useful tool for mouse lipoprotein analyses for up-coming experiments.
|
Report
(4 results)
Research Products
(29 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Taste Preference for Cholesterol-Rich Diet in Mice.2016
Author(s)
Tsujita M, Ugawa S, Kobayashi F, Yamamoto J, Hakamata Y, Ito S, Shimada S, Yokoyama S
Organizer
Vanderbilt Cardiovascular Symposium 2016. State of the Art: Cardiac and Peripheral Vascular Disease Research ,
Place of Presentation
Vanderbilt University, Nashville, TN, USA
Year and Date
2016-05-04
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
[Presentation] SR-BI functions on plasma HDL homeostasis – Low plasma preβHDL level in SR-BI null mice by 2D PAGE analysis􀀃2015
Author(s)
Maki Tsujita, Tomo Yokota, Nobukatsu Akita, Yasutaka Maekawa, Junki Yamamoto, Alan T Remaley, Shinji Yokoyama
Organizer
Arteriosclerosis, Thrombosis, and Vascular Biology Peripheral Vascular Disease (ATVB | PVD) 2015 Scientific Sessions
Place of Presentation
Hilton San Francisco Union Square Hotel, San Francisco, USA
Year and Date
2015-05-07
Related Report
-
-
