Immune complexome analysis of tissue-deposited immune complexes for application to tissue in autoimmune diseases
| Project/Area Number |
15K15373
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Nagasaki University |
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Principal Investigator |
OHYAMA Kaname 長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (50437860)
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| Co-Investigator(Kenkyū-buntansha) |
川上 純 長崎大学, 医歯薬学総合研究科(医学系), 教授 (90325639)
中村 英樹 長崎大学, 病院(医学系), 講師 (10437832)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 免疫複合体 |
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| Outline of Final Research Achievements |
We have developed "immune complexome analysis", in which ICs are separated from whole serum and then subjected to direct tryptic digestion and nano-liquid chromatography-tandem mass spectrometry. We have successfully used this method to identify disease-specific IC antigens in the sera of patients with autoimmune diseases. In this research, we aimed to develop "comprehensive analysis method of tissue-deposited immune complexes" by applying this analysis method. As a result of the optimization, it was found that reconstitution after extracting, which is less efficient than anticipated, is not advanced in antigen stimulation of metamorphic antigens in tissues. In the future, we will analyze tissue analysis of immune complexes using surface MS analysis such as liquid extraction surface analysis.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Novel anti-suprabasin antibodies may contribute to the pathogenesis of neuropsychiatric systemic lupus erythematosus.2018
Author(s)
Ichinose K, Ohyama K, Furukawa K, Higuchi O, Mukaino A, Satoh K, Nakane S, Shimizu T, Umeda M, Fukui S, Nishino A, Nakajima H, Koga T, Kawashiri SY, Iwamoto N, Tamai M, Nakamura H, Origuchi T, Yoshida M, Kuroda N, Kawakami A.
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Journal Title
Clin Immunol.
Volume: 印刷中
Pages: 123-130
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Upregulation of Thrombospondin 1 Expression in Synovial Tissues and Plasma of Rheumatoid Arthritis: Role of Transforming Growth Factor-beta1 toward Fibroblast-like Synovial Cells2015
Author(s)
Suzuki T, Iwamoto N, Yamasaki S, Nishino A, Nakashima Y, Horai Y, Kawashiri S, Y Ichinose K, Arima K, Tamai M, Nakamura H, Origuchi T, Miyamoto C, Osaki M, Ohyama K, Kuroda N, Kawakami A.
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Journal Title
Journal of Rheumatology
Volume: 42
Issue: 6
Pages: 943-947
DOI
Related Report
Peer Reviewed
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