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Structural analysis of maternal-fetal signal transmitter

Research Project

Project/Area Number 15K15405
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Embryonic/Neonatal medicine
Research InstitutionKanazawa Medical University

Principal Investigator

HATTA Toshihisa  金沢医科大学, 医学部, 教授 (20238025)

Co-Investigator(Renkei-kenkyūsha) SHIMADA Hiroki  金沢医科大学, 看護学部, 准教授 (60278108)
SIMAMURA Eriko  金沢医科大学, 医学部, 講師 (00267741)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords母胎児間インターフェイス / 胎盤 / 胎盤関門 / 白血病抑制因子 / 胎盤絨毛 / 栄養膜 / 母胎間シグナル伝達 / DOHad / DOHaD
Outline of Final Research Achievements

Histological structures which bridge between the sinusoidal space on the maternal side and vessels of placental villi on the fetal side was investigated. The glial fibrillary acidic protein (GFAP) immunostaining revealed that the GFAP-positive long fibers run across the mesenchyme in the trophoblasts. Then, immunostaining of HKX-1, the specific marker for the neural crest cells, exhibited that the numerous HKX-1-positive cells exists in the placenta. These findings suggest that glial-like cells of neural crest origin contribute to placental barriers similar to the blood-brain barrier. The RAP protocol for tissue clearing was applied to the immunostaining, showing that the RAP-treated specimens exhibited stronger signals. However, the lipophilic fluorescence tracer (DiI) for revealing the whole view of long process, could not be applied for RAP-treated specimens. Thus, in this project, whole mount imaging analysis of the placenta was almost established.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (33 results)

All 2018 2017 2016 2015

All Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 10 results,  Open Access: 7 results,  Acknowledgement Compliant: 3 results) Presentation (21 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] A rapid and nondestructive protocol for whole-mount bone staining of small fish and Xenopus2018

    • Author(s)
      Sakata-Haga H, Uchishiba M, Shimada H, Tsukada T, Mitani M, Arikawa T, Shoji H, Hatta T
    • Journal Title

      SCIENTIFIC REPORTS

      Volume: - Issue: 1 Pages: 7453-7453

    • DOI

      10.1038/s41598-018-25836-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Extracranial outfolw of particles solved in cerebrospinal fluid: Fluorescein injection study2017

    • Author(s)
      Akai1 T, Hatta T, Shimada H, Mizuki K, Kudo N, Hatta T, Otani H
    • Journal Title

      Congenital Anomalies

      Volume: - Issue: 3 Pages: 93-98

    • DOI

      10.1111/cga.12257

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] JunB regulates angiogenesis and neurovascular parallel alignment in mouse enbryonic skin2017

    • Author(s)
      Yoshitomi Y, Ikeda T, Saito H, Yoshitake Y, Ishigaki Y, Hatta T, Kato N, Yonekura H
    • Journal Title

      Journal of Cell Science

      Volume: 130 Issue: 5 Pages: 916-926

    • DOI

      10.1242/jcs.196303

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Galectin-4 expression is down-regulated in response to autophagy during differentiation of rat trophoblast cells2016

    • Author(s)
      Arikawa T, Shengjun Liao, Shimada H, Inoue T, Sakata-Haga H, Nakamura T, Hatta T Shoji H
    • Journal Title

      Scientific Reports 6

      Volume: 6 Issue: 1 Pages: 32248-32248

    • DOI

      10.1038/srep32248

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 神経管の発生2016

    • Author(s)
      八田稔久
    • Journal Title

      小児の脳神経

      Volume: 41 Pages: 296-303

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 生物試料の透明化:古典から最新技術まで2016

    • Author(s)
      八田稔久、三谷真弓
    • Journal Title

      医生電顕技術雑誌

      Volume: 29 Pages: 54-56

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 生物試料の透明化:古典から最新技術まで2016

    • Author(s)
      八田稔久、三谷真弓
    • Journal Title

      医生電顕技術誌

      Volume: 29 Pages: 54-56

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Melanocortins Contribute to Sequential Differentiation and Enucleation of Human Erythroblasts via Melanocortin Receptors 1,2 and 52015

    • Author(s)
      Simamura E, Arikawa T, Ikeda T, Shimada H, Shoji H, Masuta H, Nakajima Y, Otani H, Yonekura H, Hatta T
    • Journal Title

      PLOS ONE

      Volume: 10 Issue: 4 Pages: 1-17

    • DOI

      10.1371/journal.pone.0123232

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The suppression of maternal-fetal leukemia inhibitory factor signal relay pathway by maternal immune activation impairs brain development in mice2015

    • Author(s)
      Tsukada T, Simamura E, Shimada H, Arai T, Higashi N, Akai T, Iizuka H, Hatta T
    • Journal Title

      PLOS ONE

      Volume: 4 Issue: 6 Pages: 1-14

    • DOI

      10.1371/journal.pone.0129011

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Three-dimensional structure of seminiferous tubules in the adult mouse.2015

    • Author(s)
      4.Nakata H, Wakayama T, Sonomura T, Honma S, Hatta T, Iseki S.
    • Journal Title

      J Anat.

      Volume: 227 Issue: 5 Pages: 686-694

    • DOI

      10.1111/joa.12375

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 母体Poly (I:C) 投与による胎盤TLR3 シグナルの亢進部位の検索2018

    • Author(s)
      塚田剛史、島田ひろき、王 賀、坂田ひろみ、東海林博樹、八田稔久
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゼブラフィッシュ骨格観察のための簡易透明化法の開発と解析法の検討2017

    • Author(s)
      坂田ひろみ、島田ひろき、狩山信生, 有川智博, 東海林博樹, 八田稔久
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎大学坂本キャンパス(長崎県長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 胎児脳発達を促進する母胎間白血病抑制因子(LIF)シグナルリレー発動の検出方法の検討2017

    • Author(s)
      三浦公実、阪上大昌、島田ひろき、王 賀、塚田剛史、坂田ひろみ、廖 生俊、有川智博、東海林博樹、八田稔久
    • Organizer
      第122回日本解剖学会総会・全国学術集会2017
    • Place of Presentation
      長崎大学坂本キャンパス(長崎県長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 小型実験動物のための迅速骨染色法の開発2017

    • Author(s)
      坂田ひろみ、島田ひろき、塚田剛史、狩山信生、有川智博、東海林博樹、八田稔久
    • Organizer
      第57回日本先天異常学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 白血病抑制因子はマウス栄養膜幹細胞から副腎皮質刺激ホルモン放出ホルモンの分泌を促進する2017

    • Author(s)
      王 賀、有川智博、廖 生俊、塚田剛史、坂田ひろみ、島田ひろき、東海林博樹、八田稔久
    • Organizer
      第57回日本先天異常学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マウス母体へのLIF投与は胎児大脳介在ニューロン産生に関わる遺伝子の発現を変化させる2017

    • Author(s)
      阪上大昌、三浦公実、羽立 譲、坂田ひろみ、塚田剛史、島田ひろき、王 賀、辰野貴則、石垣靖人、八田稔久
    • Organizer
      第57回日本先天異常学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 顆粒球・マクロファージコロニー刺激因子のマウス胎仔脳組織形成における作用の解析2017

    • Author(s)
      松本暁洋、Regassa Dereje Getachew、古屋智英、小川典子、佐藤文夫、橋本龍樹、八田稔久、大谷 浩
    • Organizer
      第57回日本先天異常学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 母体Poly(I:C)投与による胎盤TLR3シグナルの亢進は脱落膜中の母体由来細胞で生じる2017

    • Author(s)
      塚田剛史、島田ひろき、王 賀、坂田ひろみ、東海林博樹、八田稔久
    • Organizer
      第77回日本解剖学会中部支部学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Immuune-edocrine communication between mother and fetus contributes to fetal brain development2016

    • Author(s)
      Hatta T, Tsukada T, Shimada H, Arikawa T, Shoji H, W He, Sakata H
    • Organizer
      Third Myanmer-Japan international Symposium
    • Place of Presentation
      Patein University(Myanmar)
    • Year and Date
      2016-12-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Autophagy contributes to the differentiation of rat trophoblast cells2016

    • Author(s)
      Shengjun Liao, Arikawa T, Shimada H, Sakata-Haga H, Hatta H, Shoji H
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Critical role of SOCS3 in the suppression of the in matenal-fetalleukemia inhibitory factor signalrelay pathway for fetal brain development2016

    • Author(s)
      Tsukada T, Shimada H, W He, Sakata-Haga H, Iizuka H, Hatta H
    • Organizer
      5th Conference on Prenatal Programming and Toxicity
    • Place of Presentation
      北九州国際会議場(福岡県小倉市)
    • Year and Date
      2016-11-13
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] バルプロ酸が脊髄神経の形成に及ぼす影響2016

    • Author(s)
      坂田ひろみ、Bold luramt、福井義浩、島田ひろき、八田稔久
    • Organizer
      第76回日本解剖学会中部支部学術集会
    • Place of Presentation
      信州大学医学部(長野県松本市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
  • [Presentation] 大脳皮質発生に関わる母胎児間LIF-ACTH-LIFシグナルリレーにおける胎盤CrhとPomcの挙動2016

    • Author(s)
      塚田剛史、島田ひろき、王 賀、東伸明、飯塚秀明、八田稔久
    • Organizer
      第56回日本先天異常学会学術集会
    • Place of Presentation
      姫路商工会議所(兵庫県姫路市)
    • Year and Date
      2016-07-29
    • Related Report
      2016 Research-status Report
  • [Presentation] マウス胎仔脳組織形成における顆粒球・マクロファージコロニー刺激因子の作用の解析2016

    • Author(s)
      松本暁洋、古屋智英、小川典子、佐藤文夫、橋本龍樹、八田稔久、大谷 浩
    • Organizer
      第56回日本先天異常学会学術集会
    • Place of Presentation
      姫路商工会議所(兵庫県姫路市)
    • Year and Date
      2016-07-29
    • Related Report
      2016 Research-status Report
  • [Presentation] 脳室内脳せき髄液とくも膜下腔脳脊髄液の動きの相違とその意義2016

    • Author(s)
      赤井卓也、八田稔久、島田ひろき、水城圭司、工藤奈江、八田泰三、大谷 浩
    • Organizer
      第56回日本先天異常学会学術集会
    • Place of Presentation
      姫路商工会議所(兵庫県姫路市)
    • Year and Date
      2016-07-29
    • Related Report
      2016 Research-status Report
  • [Presentation] GFPマウス胎仔の透明化による心臓発生過程の立体画像化2016

    • Author(s)
      吉村衣里子、杉原 諒、三浦公実、島田ひろき、東 伸明、三谷真弓、狩山信生、有川智博、東海林博樹、八田稔久
    • Organizer
      第121回日本解剖学会総会・学術集会
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 組織透明化技術2016

    • Author(s)
      八田稔久
    • Organizer
      ”未来へのバイオ技術”勉強会「ハイコンテントアナリシス(HCA)技術の進化」
    • Place of Presentation
      一般財団法人バイオインダストリー協会(東京都中央区)
    • Year and Date
      2016-02-25
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Significance of autophagy and down-regulation of Galectin-4 expression during differentiation of rat trophoblast cells2016

    • Author(s)
      廖生俊、有川智博、島田ひろき、坂田ひろみ、八田稔久、石垣靖人、東海林博樹
    • Organizer
      第52回金沢医科大学医学会学術集会
    • Place of Presentation
      金沢医科大学(石川県河北郡内灘町)
    • Related Report
      2016 Research-status Report
  • [Presentation] 母体免疫亢進時におけるインターロイキン6の母胎間移行動態2015

    • Author(s)
      島田ひろき、塚田剛史、有川智博、東海林博樹、東 伸明、八田稔久
    • Organizer
      第4回日本DOHaD研究会学術集会
    • Place of Presentation
      昭和大学(東京都品川区)
    • Year and Date
      2015-08-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 母体免疫活性化による母胎間LIF-ACTH-LIFシグナルリレーの破綻と胎仔脳形成障害2015

    • Author(s)
      塚田剛史、島村英理子、島田ひろき、東伸明、赤井卓也、飯塚秀明、八田稔久
    • Organizer
      第55回日本先天異常学会学術集会
    • Place of Presentation
      パシフィコ横浜会議センター(神奈川県横浜市)
    • Year and Date
      2015-07-25
    • Related Report
      2015 Research-status Report
  • [Presentation] 生物試料の透明化:古典から最新技術まで2015

    • Author(s)
      八田稔久
    • Organizer
      医学生物学電子顕微鏡技術学会
    • Place of Presentation
      名古屋市立大学医学部(愛知県名古屋市)
    • Year and Date
      2015-06-19
    • Related Report
      2015 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] Kit for producing cleared biological specimens and method for producing cleared biological specimens2015

    • Inventor(s)
      Toshihisa Hatta
    • Industrial Property Rights Holder
      Kanazawa Medical University
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-05-28
    • Related Report
      2015 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 透明化生物標本作製用キット及び透明化生物標本作製方法2015

    • Inventor(s)
      八田稔久
    • Industrial Property Rights Holder
      学校法人金沢医科大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-544550
    • Filing Date
      2015-04-20
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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