A noble finding of tumor invasion using scanning electron microscopy analysis based on NanoSuit

• Project/Area Number: 15K15413
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Hirakawa Satoshi 浜松医科大学, 医学部, 准教授 (50419511)
• Co-Investigator(Renkei-kenkyūsha): HARIYAMA Takahiko 浜松医科大学, 光尖端医学教育研究センター, 特任教授 (30165039) ; TAKAKU Yasuharu 浜松医科大学, 光尖端医学教育研究センター, 特任助教 (60378700) ; OHTA Isao 浜松医科大学, 光尖端医学教育研究センター, 技術専門職員 (20464133) ; ISHII Daisuke 名古屋工業大学, 工学部, 准教授 (60435625)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: がん細胞 / 電子顕微鏡 / 内皮細胞 / 浸潤 / 治療モデル / 細胞 / 形態 / 化合物 / 癌 / 細胞・組織

Outline of Final Research Achievements

Cancer cells migrate to interstitial matrix in tumor microenvironment. Furthermore, tumor cells metastasize to regional lymph nodes and/or distant organs including lung. Importantly, those cells migrate in tumor-associated lymphatic vessels, and are drained to the lymph nodes, leading to the formation of metastatic foci by the tumor cells. However, little is known about the process how tumor cells infiltrate and migrate in the lymphatic endothelial cells. Here we demonstrated the process by subjecting the materials to an electron microscopy analysis using NanoSuit, which is a nano-sized membrane polymerized on biomaterials including cell membrane. A375, a cell line from human malignant melanoma, interacts with mesenchymal cells such as endothelial cells. Therefore, we developed a three-dimensional tissue culture system to demonstrate lymphatic endothelial cells with A375 by scanning electron microscopy, and successfully found that those cells interact in a living condition.

Research Products

• [Journal Article] A modified ‘NanoSuit’ preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy. (2017)
  • Author(s): Takaku Y, Suzuki H, Kawasaki H, Ohta I, Ishii D, Hirakawa S, Tsutsui T, Matsumoto H, Takehara S, Nakane C, Sakaida K, Suzuki C, Muranaka Y, Kikuchi H, Konno H, Shimomura M, Hariyama T
  • Journal Title: R Soc Open Sci Volume: なし Issue: 3 Pages: 160887-160887
  • DOI: 10.1098/rsos.160887
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Development of full-thickness human skin equivalens with blood and lymph-like capillary networks by cell coating technology (2015)
  • Author(s): Matsusaki M, Fujimoto K, Shirakata Y, Hirakawa S, Hashimoto K, Akashi M.
  • Journal Title: J Biomed Mater Res A Volume: 印刷中 Issue: 10 Pages: 3386-3396
  • DOI: 10.1002/jbm.a.35473
  • Peer Reviewed
• [Presentation] EGFRiによる皮膚障害:ナノスーツ法を応用した新たな評価法 (2015)
  • Author(s): 平川聡史、高久康春、太田 勲、石井大佑、針山孝彦
  • Organizer: 第53回日本癌治療学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2015-10-29
• [Presentation] ナノスーツ法: 分子標的薬による皮膚障害を評価する新たな診断技術 (2015)
  • Author(s): 平川聡史、高久康春、太田 勲、石井大佑、針山孝彦
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Patent(Industrial Property Rights)] 含水状態の生物試料の電子顕微鏡観察用保護剤、電子顕微鏡観察用キット、電子顕微鏡による観察、診察、評価の方法並びに試料台 (2015)
  • Inventor(s): 針山孝彦、高久康春、平川聡史、河崎秀陽
  • Industrial Property Rights Holder: 針山孝彦、高久康春、平川聡史、河崎秀陽
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-04-01
  • Overseas