Transgenelation of behavioral abnormaliteis associated with DNA methylation signatures in sperm

• Project/Area Number: 15K15425
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Psychiatric science
• Research Institution: University of Fukui
• Principal Investigator: MATSUZAKI Hideo 福井大学, 子どものこころの発達研究センター, 教授 (00334970)
• Co-Investigator(Kenkyū-buntansha): 岩田 圭子 福井大学, 子どものこころの発達研究センター, 助教 (30415088) ; 小西 慶幸 福井大学, 学術研究院工学系部門, 准教授 (00382838)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 母体ストレス / マウス / 行動異常 / 遺伝子発現 / 精子 / エピジェネティックマーク

Outline of Final Research Achievements

In this study, we find that maternal stress influences behavior in subsequent generations. In addition, some gene expressions involved in the abnormal behaviors are changed in brains of subsequent generations. We also find that methylation status of these gene in sperm DNA is changed in subsequent generations. From these results, transgenelation of behavioral abnormalities associated with DNA methylation in sperm is suggested. In the future, detailed experiments of DNA methylation in sperm is needed.

Research Products

• [Int'l Joint Research] University of Padova(Italy)
• [Journal Article] Microglia-derived neuregulin expression in psychiatric disorders. (2017)
  • Author(s): Ikawa D, Makinodan M, Iwata K, Ohgidani M, Kato TA, Yamashita Y, Yamamuro K, Kimoto S, Toritsuka M, Yamauchi T, Fukami SI, Yoshino H, Okumura K, Tanaka T, Wanaka A, Owada Y, Tsujii M, Sugiyama T, Tsuchiya K, Mori N, Hashimoto R, Matsuzaki H, Kanba S, Kishimoto T.
  • Journal Title: Brain Behav Immun. Volume: 61 Pages: 375-385
  • DOI: 10.1016/j.bbi.2017.01.003
  • Peer Reviewed / Open Access
• [Journal Article] Tumor necrosis factor-alpha expression in peripheral blood mononuclear cells correlates with early childhood social interaction in autism spectrum disorder. (2017)
  • Author(s): Makinodan M, Iwata K, Ikawa D, Yamashita Y, Yamamuro K, Toritsuka M, Kimoto S, Okumura K, Yamauchi T, Yoshino H, Tsujii M, Sugiyama T, Tsuchiya K, Mori N, Matsuzaki H, Kishimoto T.
  • Journal Title: Neurochem Int. Volume: 104 Pages: 1-5
  • DOI: 10.1016/j.neuint.2016.12.005
  • Peer Reviewed
• [Journal Article] ARPP-16 is a striatal-enriched inhibitor of protein phosphatase 2A regulated by microtubule-associated serine/threonine kinase 3. (2017)
  • Author(s): Andrade E, Musante V, Horiuchi A, Matsuzaki H, Brady A, Wu T, Greengard P, Taylor J, and Nairn A.
  • Journal Title: J Neurosci. Volume: 37(10) Issue: 10 Pages: 2709-2722
  • DOI: 10.1523/jneurosci.4559-15.2017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Mismatch in cerebral blood flow and glucose metabolism after the forced swim stress in rats. (2016)
  • Author(s): Kameno Y, Suzuki K, Takagai S, Iwata K, Matsuzaki H, Takahashi K, Wakuda T, Iwata Y, Magata Y, Mori N.
  • Journal Title: Acta Neuropsychiatrica. Volume: 20 Issue: 6 Pages: 1-5
  • DOI: 10.1017/neu.2016.24
  • Peer Reviewed
• [Journal Article] Fetal Environment and Glycosylation Status in Neonatal Cord Blood: A Comprehensive Mass Spectrometry-based Glycosylation Analysis. (2016)
  • Author(s): Sato R, Tsuchiya KJ, Matsuzaki H, Takei N, Itoh H, Kanayama N, Suda T, Watanabe H, Ohashi T, Tanaka M, Nishimura S, Maekawa M, HBC study group
  • Journal Title: Medicine (Baltimore). Volume: 95(14) Issue: 14 Pages: e3219-e3219
  • DOI: 10.1097/md.0000000000003219
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines. (2016)
  • Author(s): Mundalil Vasu M, Anitha A, Takahashi T, Thanseem I, Iwata K, Asakawa T, Suzuki K.
  • Journal Title: PLoS ONE Volume: 11 Issue: 10 Pages: e0164425-e0164425
  • DOI: 10.1371/journal.pone.0164425
  • Peer Reviewed / Open Access
• [Presentation] Exploring the role of mitochondrial dynamics in developmental and psychiatric disorders. (2017)
  • Author(s): Iwata K and Scorrano L
  • Organizer: 15th VIMM Annual Retreat
  • Place of Presentation: University of Padova, Padova, Italy
  • Year and Date: 2017-02-17
  • Int'l Joint Research
• [Presentation] Measurement of Multiple Radical Scavenging Activity as a diagnostic method for autism spectrum disorder in children. (2016)
  • Author(s): Aki Hirayama, Matsuzaki H.
  • Organizer: The Society for Redox Biology and Medicine’s 23rd Annual Meeting
  • Place of Presentation: Hyatt Regency San Francisco, San Francisco, USA
  • Year and Date: 2016-11-16
  • Int'l Joint Research
• [Presentation] Specific Hypolipidemia caused by VLDL degradation in Children with ASD. (2016)
  • Author(s): Matsuzaki H, Iwata K, Nakamura K, Tsujii M and Mori N.
  • Organizer: The Society for Neuroscience 46th annual meeting
  • Place of Presentation: San Diego Convention Center, San Diego, USA
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] Genome-wide DNA methylation profiles in postmortem brains from subjects with autism. (2016)
  • Author(s): Iwata K, Nakabayashi K, Hata K, Nakamura K, N Shintani, Matsuzaki H, Mori N.
  • Organizer: The Society for Neuroscience 46th annual meeting
  • Place of Presentation: San Diego Convention Center, San Diego, USA
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] 自閉症者血清中の糖脂質: MALDI-TOF- MSによる網羅的糖鎖分析． (2016)
  • Author(s): 原田陽子、渡邊賢、松﨑秀夫
  • Organizer: 第38回日本生物学的生物精神医学会・第59回日本神経化学会合同年会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-09-08
• [Presentation] 自閉症死後脳縫線核メチル化状態の網羅的解析． (2016)
  • Author(s): 松﨑秀夫、岩田圭子、中林一彦、中村和彦、秦健一郎、森則夫
  • Organizer: 第38回日本生物学的生物精神医学会・第59回日本神経化学会合同年会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-09-08
• [Presentation] NSF interacts with SERT and modulates its trafficking: implications for pathophysiology in autism． (2016)
  • Author(s): 松﨑秀夫
  • Organizer: 第５回日本DOHaD研究会年会
  • Place of Presentation: 国立成育医療研究センター講堂（東京都世田谷区）
  • Year and Date: 2016-07-23
• [Presentation] Clinical research for Child Mental Development in Japan. (2016)
  • Author(s): Matsuzaki H
  • Organizer: VIMM seminar
  • Place of Presentation: University of Padova, Padova, Italy
  • Int'l Joint Research / Invited