Electrochemical concentration of radiometals in the microspace and its application to labeling of peptid

• Project/Area Number: 15K15444
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Tohoku University
• Principal Investigator: Iwata Ren 東北大学, サイクロトロン・ラジオアイソトープセンター, 名誉教授 (60143038)
• Co-Investigator(Kenkyū-buntansha): 高橋 和弘 秋田県立脳血管研究センター(研究部門), その他部局等, 研究員 (20370257)
• Co-Investigator(Renkei-kenkyūsha): ISHIKAWA Yoichi 東北大学, サイクロトロン・ラジオアイソトープセンター, 助手 (60361200)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 放射性金属 / 電気化学的濃縮 / ペプチド・抗体標識 / 標識技術 / 放射性金属核種 / 電極反応 / ペプチド・タンパク質標識

Outline of Final Research Achievements

The present study was aimed to develop a challenging method for separating radioactive metal ions in a small space by electrochemical adsorption and desorption on the Pt/carbon electrode and for labeling peptides or antibodies with recovered radioactive metals by chelating reactions. [18F]Fluoride was concentrated and then converted in >75% to A][18F]2+ with Al3+ in the narrow channel of an electrochemical chip. Recovery yields, however, were varied independently on applied voltage and time probably due to the surface conditions of the carbon electrode. A custom-made target chamber was developed and successfully tested for producing 64Cu by the 64Ni(p,n)64Cu reaction with the HM12 cyclotron at CYRIC. Transtuzumab and Cetuximab were chosen as a candidate antibody. They were modified with DOTA-NHS-ester as a chelating agent and a protocol suitable for the preparation of 64Cu-DOTA-Transtuzumab and 64Cu-DOTA-Cetuximab in a small scale was established.

Research Products

• [Journal Article] Continuous-flow synthesis of N-succinimidyl 4-[18F]fluorobenzoate using a single microfluidic chip. (2016)
  • Author(s): Kimura H., Tomatsu K., Saiki H., Arimitsu K., Ono M., Kawashima H., Iwata R., Nakanishi H., Ozeki E., Kuge Y., Saji H.
  • Journal Title: Plos One Volume: 11 Issue: 7 Pages: e0159303-e0159303
  • DOI: 10.1371/journal.pone.0159303
  • NAID: 120005997759
  • Peer Reviewed
• [Journal Article] Synthesis and characterization of 18F-interleukin-8 using a cell-free translation system and 18F-fluoro-L-proline, Journal of Nuclear Medicine (2016)
  • Author(s): Ryuichi Harada, Shozo Furumoto, Takeo Yoshikawa, Yoichi Ishikawa, Katsutoshi Shibuya, Nobuyuki Okamura, Kiichi Ishiwata, Ren Iwata, Kazuhiko Yanai
  • Journal Title: Journal of Nuclear Medicine Volume: 57(4) Issue: 4 Pages: 634-639
  • DOI: 10.2967/jnumed.115.162602
  • Peer Reviewed
• [Journal Article] High-uptake areas on positron emission tomography with the hypoxic radiotracer 18F-FRP170 in glioblastomas include regions retaining proliferative activity under hypoxia. (2015)
  • Author(s): Beppu T, Sasaki T, Terasaki K, Saura H, Mtsuura H, Ogasawara K, Sasaki M, Ehara S, Iwata R, Takai Y
  • Journal Title: Ann Nucl Med Volume: 29 Issue: 4 Pages: 336-341
  • DOI: 10.1007/s12149-015-0951-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Quantitative kinetic analysis of PET amyloid imaging agents [(11)C]BF227 and [(18)F]FACT in human brain. (2015)
  • Author(s): Shidahara M, Watabe H, Tashiro M, Okamura N, Furumoto S, Watanuki S, Furukawa K, Arakawa Y, Funaki Y, Iwata R, Gonda K, Kudo Y, Arai H, Ishiwata K, Yanai K.
  • Journal Title: Nucl Med Biol. Volume: 42 Issue: 9 Pages: 734-744
  • DOI: 10.1016/j.nucmedbio.2015.05.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Preclinical evaluation of [18F]THK-5015 enantiomers: effects of chirality on its effectiveness as a tau imaging radiotracer. (2015)
  • Author(s): Tago T., Furumoto S., Okamura N., Harada R., Adachi H., Ishikawa Y., Yanai K., Iwata R., Kudo Y.
  • Journal Title: Mol. Imaging Biol. Volume: 未定 Issue: 2 Pages: 258-266
  • DOI: 10.1007/s11307-015-0879-8
  • Peer Reviewed
• [Journal Article] [18F]THK-5117 PET for assessing neurofibrillary pathology in Alzheimer's disease. (2014)
  • Author(s): Harada R, Furukawa K, et al.
  • Journal Title: Eur J Nucl Med Mol Imaging Volume: 42 Issue: 7 Pages: 1052-1061
  • DOI: 10.1007/s00259-015-3035-4
  • Peer Reviewed / Acknowledgement Compliant