| Project/Area Number |
15K15520
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中山 若樹 北海道大学, 医学研究科, 講師 (40421961)
数又 研 北海道大学, 大学病院, 講師 (60634144)
鐙谷 武雄 北海道大学, 大学病院, 助教 (80270726)
七戸 秀夫 北海道大学, 大学病院, 准教授 (80374479)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | もやもや病 / iPS細胞 / 平滑筋細胞 / 血管平滑筋細胞 / 神経堤 / 神経堤細胞 |
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| Outline of Final Research Achievements |
To investigate the pathogenesis of moyamoya disease, we induced smooth muscle cells from iPS cells derived from moyamoya disease patients. First, we induced neural crest cells from iPS cells. We found more than 99% of the cells were positive for p75. Next we differentiated these neural crest cells into smooth muscle cells, however, the cells become senescent during differentiation. Although surviving cells were almost positive for α-SMA and SM22, these cells could not be used for next analysis because of cell senescence. Improvement of the protocol is necessary to prevent cell senescence in the future.
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