Serum diagnosis of brain tumor using NMR metabolomics

• Project/Area Number: 15K15531
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Nippon Medical School
• Principal Investigator: Adachi Koji 日本医科大学, 医学部, 准教授 (00231928)
• Co-Investigator(Kenkyū-buntansha): 平川 慶子 日本医科大学, 医学部, 助教 (30165162)
• Research Collaborator: KANAWAKU Yoshimasa ; OHNO Youkichi ; KOIKE Kaoru ; MORITA Akio
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 脳腫瘍 / 血清診断 / NMR メタボロミクス / メタボロミクス解析 / NMR

Outline of Final Research Achievements

We started our research on brain tumor serum diagnosis aiming to apply NMR metabolomics techniques from 2015.　We have collected serum samples from 41 patients of brain diseases including brain tumor. Samples were measured by unique method developed by Hirakawa, who is a member of this research project, and others, and analytical technique of metabolomics. As a result, we cannot clearly distinguish glioma group from non-gliomatous brain tumors and nontumoral brain diseases clearly in this approach.

Academic Significance and Societal Importance of the Research Achievements

脳腫瘍の診断が血清で可能となると、疾病のモニタリングや健康人に対するスクリーニングに威力を発揮すると考えられます。今回の研究では、脳腫瘍の血清診断をめざし、脳腫瘍を含む41例の脳疾患症例からの血液検体を NMR メタボロミクスの技術で解析しました。結果としては、この手法では脳腫瘍と非腫瘍性脳疾患の区別が十分にはできませんでした。現在この技術を改良した手法で研究を続けています。