Investigation of effects of anesthetics on phenotype and gene expression of cancer cells with CTOS method
| Project/Area Number |
15K15577
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
IWAI Teppai 関西医科大学, 医学部, 助教 (90440966)
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| Co-Investigator(Kenkyū-buntansha) |
広田 喜一 関西医科大学, 医学部, 教授 (00283606)
奥山 裕照 地方独立行政法人大阪府立病院機構大阪国際がんセンター(研究所), その他部局等, 総括研究員 (50432373)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 麻酔薬 / がん細胞 / 悪性度 / CTOS / HIF-1 / RNA-Seq / がん / 転移 / スフェロイド |
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| Outline of Final Research Achievements |
Growing evidence indicates that perioperative factors, including choice of anesthetic, affect cancer recurrence after surgery although little is known about the effect of anesthetics on cancer cells themselves. Certain anesthetics are known to affect phenotypes of cells derived from cancer. In this study, we used cells established from cancer tissue and the primary culture cells from the cancer tissue-originated spheroid (CTOS) method.
Our results provide evidence that the anesthetics isoflurane and sevoflurane did not exert a pro-tumorigenic effect on human cancer cell lines and spheroid cells by CTOS. We present evidence that the anesthetics did not affect phenotypes of cells derived from cancer, cell signaling pathway involving in tumorigenesis nor gene expression patterns associated with a malignant phenotype.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] Dynamic Change of Polarity in Primary Cultured Spheroids of Human Colorectal Adenocarcinoma and Its Role in Metastasis2016
Author(s)
Okuyama H, Kondo J, Sato Y, Endo H, Nakajima A, Piulats JM, Tomita Y, Fujiwara T, Itoh Y, Mizoguchi A, Ohue M, Inoue M
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Journal Title
Am J Pathol
Volume: 186
Issue: 4
Pages: 899-911
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] UCHL1 provides diagnostic and antimetastatic strategies due to its deubiquitinating effect on HIF-1alpha2015
Author(s)
Goto Y, Zeng L, YeomCJ, Zhu Y, Morinibu A, Shinoyam K, Kobayashi M, Hirota K, Itasaka S, Yoshimura M, Tanimoto K, Sowa T, Menju T, Sonobe M, Kakeya H, Toi M, Date H, Hammond EM, Hiraoka M, Harada H.
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Journal Title
Nat Commun
Volume: 6
Issue: 1
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access
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