| Project/Area Number |
15K15607
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
ikawa masahito 大阪大学, 微生物病研究所, 教授 (20304066)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | ゲノム編集 / レンチウイルス / 実験動物 / 遺伝子改変 / 妊娠高血圧症候群 / レンチウイルスベクター / 胎盤 / 高血圧 |
|---|
| Outline of Final Research Achievements |
Placentation is a critical event for bot maternal and fetal life. In the present study, we utilized CRISPR/Cas9 system and lentiviral vector mediated placenta specific transgenic system to identify the genes important for placentation and decipher their roles. By injecting sgRNA/Cas9 expressing plasmids, we have knocked out 6 placenta specific genes and identified 1 gene critical for embryonic development. On the other hand, we overexpressed PlacI and Elf5 in placenta specifically and found these genes play critical roles during placentaion and fetal development.
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