Novel biomaker for head and neck cancer in exosome

Research Project

Project/Area Number	15K15617
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Otorhinolaryngology
Research Institution	Kobe University
Principal Investigator	Ken-ichi Nibu 神戸大学, 医学研究科, 教授 (20251283)
Co-Investigator(Kenkyū-buntansha)	大月直樹神戸大学, 医学研究科, 准教授 (40343264)
Co-Investigator(Renkei-kenkyūsha)	Takeshi Fujita 近畿大学, 医学部, 講師 (90533711)
Research Collaborator	Natsumi Uehara 神戸大学, 医学部附属病院, 助教 (40570502)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	エクソソーム / マイクロRNA / 頭頸部癌 / バイオマーカー
Outline of Final Research Achievements	Technical procedures of exosome isolation and microRNA extraction from head and neck cancer cell lines were established. Based on these techniques, protocol of clinical research to identify novel biomarker for head and neck cancer was prepared and approved by Kobe University ethical committee. In this research, tissues of tumor and bloods from draining vein of tumor as well as tissues of contralateral normal organ and bloods from draining vein of contralateral normal organ are harvested. Exosomes are extracted and microRNAs are extracted from these materials. Results will be analyzed to identify novel biomarkers for head and neck cancer. Our results will contribute to early diagnosis of head and neck cancer and lead to development of gene therapy using replication-selective adenoviral vector with specific promotor for head and neck cancer.