| Project/Area Number |
15K15646
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatric surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Tajiri Tatsuro 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80304806)
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| Co-Investigator(Kenkyū-buntansha) |
岸田 綱郎 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00370205)
木村 修 京都府立医科大学, 医学(系)研究科(研究院), 特任教授 (10315963)
東 真弓 京都府立医科大学, 医学(系)研究科(研究院), 助教 (10380453)
文野 誠久 京都府立医科大学, 医学(系)研究科(研究院), 助手 (40405254)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 再生医学 / 小児外科 / 先天性横隔膜ヘルニア / 骨格筋 / 臍帯血 / 細胞工学 / 幹細胞 / 小児外科学 / 発生学 |
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| Outline of Final Research Achievements |
Diaphragmatic regeneration using umbilical cord blood derived stem cels for diaphragmatic agenesis was attempted using 3 types of different stem cells. Connection of small muscle sphere was not observed. Therefore, we attempted direct conversion from human fibroblast into myoblast using MyoD1 and L-myc gene trasfection as next step. Finally, we obtained
multinucleated muscle cells with high efficiency. we perspective function myosheet using this novel technique.
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