Epigenetic change when fibroblasts become undifferenciated

• Project/Area Number: 15K15656
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Keio University
• Principal Investigator: Kishi Kazuo 慶應義塾大学, 医学部(信濃町), 教授 (40224919)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: epigenetic / regeneration / 脱分化 / 皮膚 / 再生 / エピジェネティックス

Outline of Final Research Achievements

By using fibroblasts, we cultured in adhesive condition using ordinary 2D culture and non-adhesive 3D condition using non-adhesive culture dishes. After the conditions except for adhesion and non-adhesion were adjusted together, we comprehensively observed the differences in DNA methylation of the two condition. As a results, we found various DNA methylated sites in two dimensional culture. From these results, it was supposed that the change into undifferentuated state by culturing on non-adhesive dish comes from epigenetic changes.