| Project/Area Number |
15K15683
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
SAEKI Makio 新潟大学, 医歯学系, 教授 (30273692)
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| Co-Investigator(Kenkyū-buntansha) |
柿原 嘉人 新潟大学, 医歯学系, 助教 (40379938)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIZAWA Michiko 松本歯科大学, 歯学部, 教授 (60303137)
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| Research Collaborator |
AKIBA Yosuke 新潟大学, 医歯学総合病院, 講師 (70547512)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 骨再生 / 破骨細胞 / 骨吸収 / NFAT / RANKL / 骨 |
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| Outline of Final Research Achievements |
Small molecule compounds that potently affect osteoclastogenesis could be useful as chemical probes for elucidating the mechanisms of various biological phenomena, and as effective therapeutic strategies against bone resorption. An osteoclast progenitor cell-based high-throughput screening system was designed to target activation of nuclear factor of activated T cells (NFAT), which is a key event for osteoclastogenesis. Chemical compound library screening using this system identified kenpaullone, to be an NFAT regulator in osteoclasts. Kenpaullone promoted receptor activator for NF-κB ligand (RANKL)-induced NFAT activity in RAW264.7 cells and also promoted the formation of TRAP-positive multinucleated osteoclasts from RAW264.7 cells and mouse primary bone marrow macrophages (BMMs) in a concentration-dependent manner.
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