Did calcitonine appear as a central nerve hormone?

• Project/Area Number: 15K15688
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: Udagawa Nobuyuki 松本歯科大学, 歯学部, 教授 (70245801)
• Co-Investigator(Kenkyū-buntansha): 小林 泰浩 松本歯科大学, 総合歯科医学研究所, 教授 (20264252) ; 山下 照仁 松本歯科大学, 総合歯科医学研究所, 准教授 (90302893) ; 上原 俊介 松本歯科大学, 歯学部, 講師 (90434480)
• Research Collaborator: YAMAUCHI Hirose ; OKABE Koji ; TAKAHASHI Naoyuki ; THIRUKONDA Gnanasagar
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: カルシトニン / 破骨細胞 / 骨吸収 / カモノハシ / オポッサム / アクチンリング / カルシトニン受容体

Outline of Final Research Achievements

Calcitonin peptides of non-mammalian species exhibit stronger activity than those of mammals. We herein determined whether platypus and opossum calcitonins exhibit similar biological activities to those of non-mammalian calcitonins using an assay of actin ring formation in mouse osteoclasts. Consistent with the strong similarities in their primary amino acid sequences, platypus and opossum calcitonins disrupted actin rings with similar efficacies to that of salmon calcitonin. Human calcitonin exhibited the weakest inhibitory potency and required a 100-fold higher concentration than that of salmon calcitonin. Platypus and opossum calcitonins also induced cAMP production in osteoclast cultures with the same efficacies as that of salmon calcitonin. These results suggest that platypus and opossum calcitonins are classified into the salmon-type group, in terms of the biological activities and amino acid sequences.

Research Products

• [Journal Article] Platypus and opossum calcitonins exhibit strong activities, even though they belong to mammals. (2017)
  • Author(s): Yamashita T, Udagawa N, Thirukonda GJ, Uehara S, Yamauchi H, Suzuki N, Li F, Kobayashi Y, Takahashi N.
  • Journal Title: Gen Comp Endocrinol. Volume: 246 Pages: 270-278
  • DOI: 10.1016/j.ygcen.2017.01.001
  • Peer Reviewed / Open Access
• [Journal Article] Wnt16 regulates osteoclast differentiation in conjunction with Wnt5a. (2015)
  • Author(s): Kobayashi Y, Thirukonda GJ, Nakamura Y, Koide M, Yamashita T, Uehara S, Kato H, Udagawa N, Takahashi N
  • Journal Title: Biochem Biophys Res Commun Volume: 463 Issue: 4 Pages: 1278-1283
  • DOI: 10.1016/j.bbrc.2015.06.102
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The dynamin inhibitor dynasore inhibits bone resorption by rapidly disrupting actin rings of osteoclasts. (2015)
  • Author(s): Thirukonda GJ, Uehara S, Nakayama T, Yamashita T, Nakamura Y, Mizoguchi T, Takahashi N, Yagami K, Udagawa N, Kobayashi Y
  • Journal Title: J Bone Miner Metab Volume: -
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] High-performance scaffolds on titanium surfaces: Osteoblast differentiation and mineralization promoted by a globular fibrinogen layer through cell-autonomous BMP signaling. (2015)
  • Author(s): Horasawa N, Yamashita T, Uehara S, Udagawa N
  • Journal Title: Mater Sci Eng C Mater Biol Appl Volume: 46 Pages: 86-96
  • DOI: 10.1016/j.msec.2014.10.025
  • Peer Reviewed
• [Journal Article] Dendritic cell-based immunotherapy targeting Wilms' Tumor 1 (WT1) in patients with relapsed malignant glioma. (2015)
  • Author(s): Sakai K, Shimodaira S, Maejima S, Udagawa N, Sano K, Higuchi Y, Koya T, Ochiai T, Koide M, Uehara S, Nakamura M, Sugiyama H, Yonemitsu Y, Okamoto M, Hongo K.
  • Journal Title: J Neurosurg Volume: 123 Issue: 4 Pages: 989-997
  • DOI: 10.3171/2015.1.jns141554
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparing immunocompetent and immunodeficient mice as ectopic transplantation animal models for bone tissue engineering (2015)
  • Author(s): Zhang Y, Li X, Chihara T, Mizoguchi T, Hori A, Udagawa N, Takahashi N, Nakamura H, Hasegawa H, Taguchi A, Shinohara A, Kagami H
  • Journal Title: Oral Diseases Volume: 21 Issue: 5 Pages: 583-592
  • DOI: 10.1111/odi.12319
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 有袋類や単孔類のカルシトニンは強力な生物活性を持つ (2017)
  • Author(s): 山下照仁，宇田川信之，山内広世，鈴木信雄，上原俊介，小林泰浩，高橋直之
  • Organizer: 日本骨代謝学会学術集会 (第35回)
  • Place of Presentation: ホテル日航福岡(福岡県)
  • Year and Date: 2017-07-27
• [Presentation] A RANKL-binding peptide W9 inhibits human osteoclast differentiation and stimulates human osteoblast differentiation (2015)
  • Author(s): Nakamura M, Nakamichi Y, Yamashita T, Furuya Y, Yasuda H, Udagawa N
  • Organizer: 米国骨代謝学会（ASBMR）
  • Place of Presentation: ワシントンステートコンベンションセンター（米国ワシントン州）
  • Year and Date: 2015-10-11
  • Int'l Joint Research
• [Presentation] Rho-Pkn3 Pathway Regulates the Bone-resorbing Activity of Osteoclasts under Wnt5a-Ror2 (2015)
  • Author(s): Uehara S, Mukai H, Yamashita T, Nakamura T, Kato S, Kikuchi A, Nishita M, Minami Y, Udagawa N, Takahashi N, Kobayashi Y
  • Organizer: 米国骨代謝学会（ASBMR）
  • Place of Presentation: ワシントンステートコンベンションセンター（米国ワシントン州）
  • Year and Date: 2015-10-10
  • Int'l Joint Research
• [Presentation] 骨は生きている-骨吸収と骨形成のカップリング機構- (2015)
  • Author(s): 宇田川信之
  • Organizer: 第58回秋季日本歯周病学会学術大会
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
  • Year and Date: 2015-09-12
  • Invited
• [Presentation] 骨代謝カップリング機構の新展開 骨のカップリングにおけるOPGの重要性 (2015)
  • Author(s): 宇田川信之
  • Organizer: 第33回日本骨代謝学会サテライトシンポジウム
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-07-25
  • Invited
• [Presentation] オステオプロテゲリン結合サイトカインであるTRAILの骨組織における役割 (2015)
  • Author(s): 宇田川信之, 中村美どり，中道裕子，小出雅則，小林泰浩，高橋直之
  • Organizer: 日本骨免疫学会（第１回）
  • Place of Presentation: ブリーズベイマリーナ（沖縄県宮古島市）
  • Year and Date: 2015-06-30
• [Book] 骨ﾍﾟﾃﾞｨｱ 骨疾患・骨代謝キーワード辞典 (2015)
  • Author(s): 宇田川信之、小林泰浩
  • Total Pages: 327
  • Publisher: 羊土社