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Development of optimally cross-linked collagen scaffold directed to differentiation control of stem cells

Research Project

Project/Area Number 15K15704
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Prosthodontics/ Dental materials science and
Research InstitutionNiigata University

Principal Investigator

Kaku Masaru  新潟大学, 医歯学系, 准教授 (30547542)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsコラーゲン架橋 / 幹細胞 / 移植担体 / 骨芽細胞 / 間葉系幹細胞 / 架橋阻害剤 / 歯根膜細胞
Outline of Final Research Achievements

This study is based on the idea that control of collagen cross-linking in collagen-based cell transplantation carrier not only changes the mechanical properties of the material but also can promote osteoblast differentiation of mesenchymal stem cells. The aim of this project was to analyze the effect of collagen crosslinking on osteoblast differentiation of mesenchymal stem cells. The addition of a crosslinking inhibitor in the osteoblast culture system under certain conditions has succeeded in selectively reducing collagen crosslinking without affecting the amount of collagen produced. Furthermore, it was revealed that changes in collagen crosslinking affect proliferation, initial adhesion, and osteoblast differentiation of mesenchymal stem cells.

Academic Significance and Societal Importance of the Research Achievements

本研究ではコラーゲン架橋の変化が間葉系幹細胞の骨芽細胞分化に影響を及ぼすことを明らかにした。幹細胞の分化制御には多様な因子が影響を及ぼすことが知られているが、細胞外微小環境としてのコラーゲン、中でもその分子間架橋構造による分化制御は新たな知見である。架橋の変化による幹細胞の分化制御機構は、再生医療に広く使われるコラーゲンを基材とした移植担体や補填材の開発への応用が期待される。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (15 results)

All 2019 2018 2017 2016 2015 Other

All Int'l Joint Research (4 results) Journal Article (11 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 11 results,  Open Access: 6 results,  Acknowledgement Compliant: 4 results)

  • [Int'l Joint Research] Univ. of North Carolina at Chapel Hill(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of North Carolina/University of Texas at Houston(米国)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] University of North Carolina/University of Texas(米国)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] University of North Carolina(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] IFT20 is required for the maintenance of cartilaginous matrix in condylar cartilage2019

    • Author(s)
      Kitami Megumi、Yamaguchi Hiroyuki、Ebina Masayuki、Kaku Masaru、Chen Di、Komatsu Yoshihiro
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 509 Issue: 1 Pages: 222-226

    • DOI

      10.1016/j.bbrc.2018.12.107

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Extracellular matrix with defective collagen cross-linking affects the differentiation of bone cells2018

    • Author(s)
      Ida Takako、Kaku Masaru、Kitami Megumi、Terajima Masahiko、Rosales Rocabado Juan Marcelo、Akiba Yosuke、Nagasawa Masako、Yamauchi Mitsuo、Uoshima Katsumi
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 9 Pages: 1-18

    • DOI

      10.1371/journal.pone.0204306

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A multi-factorial analysis of bone morphology and fracture strength of rat femur in response to ovariectomy2018

    • Author(s)
      Rosales Rocabado Juan Marcelo、Kaku Masaru、Nozaki Kosuke、Ida Takako、Kitami Megumi、Aoyagi Yujin、Uoshima Katsumi
    • Journal Title

      Journal of Orthopaedic Surgery and Research

      Volume: 13 Issue: 1 Pages: 318-328

    • DOI

      10.1186/s13018-018-1018-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] VWC2 Increases Bone Formation Through Inhibiting Activin Signaling2018

    • Author(s)
      Almehmadi Ahmad、Ohyama Yoshio、Kaku Masaru、Alamoudi Ahmed、Husein Dina、Katafuchi Michitsuna、Mishina Yuji、Mochida Yoshiyuki
    • Journal Title

      Calcified Tissue International

      Volume: 103 Issue: 6 Pages: 663-674

    • DOI

      10.1007/s00223-018-0462-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] BRCA1 and BRCA2 tumor suppressors in neural crest cells are essential for craniofacial bone development2018

    • Author(s)
      Kitami Kohei、Kitami Megumi、Kaku Masaru、Wang Bin、Komatsu Yoshihiro
    • Journal Title

      PLOS Genetics

      Volume: 14 Issue: 5 Pages: e1007340-e1007340

    • DOI

      10.1371/journal.pgen.1007340

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An ENU-induced splice site mutation of mouse Col1a1 causing recessive osteogenesis imperfecta and revealing a novel splicing rescue.2017

    • Author(s)
      Tabeta K, Du X, Arimatsu K, Yokoji M, Takahashi N, Amizuka N, Hasegawa T, Crozat K, Maekawa T, Miyauchi S, Matsuda Y, Ida T, Kaku M, Hoebe K, Ohno K, Yoshie H, Yamazaki K, Moresco EMY, Beutler B
    • Journal Title

      Sci. Rep.

      Volume: 7(1) Issue: 1 Pages: 11717-11717

    • DOI

      10.1038/s41598-017-10343-9

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Functional Diversity of Ciliary Proteins in Bone Development and Disease2017

    • Author(s)
      Kaku M, Komatsu Y
    • Journal Title

      Curr Osteoporos Rep

      Volume: Epub ahead of print Issue: 2 Pages: 96-102

    • DOI

      10.1007/s11914-017-0351-6

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A paradigm shift for bone quality in dentistry: A literature review2017

    • Author(s)
      Kuroshima Shinichiro, Kaku Masaru, Ishimoto Takuya Sasaki Muneteru, Nakano Takayoshi, Sawase Takashi
    • Journal Title

      Journal of Prosthodontic Research

      Volume: 61 Issue: 4 Pages: 353-362

    • DOI

      10.1016/j.jpor.2017.05.006

    • NAID

      130006198474

    • ISSN
      1883-1958, 1883-9207
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recruitment of bone marrow-derived cells to the periodontal ligament via the stromal cell-derived factor- 1/C-X-C chemokine receptor type 4 axis2017

    • Author(s)
      Kaku M, Kitami M, Rosales Rocabado JM, Ida T, Akiba Y, Uoshima K.
    • Journal Title

      J Periodont Res

      Volume: Epub ahead of print Issue: 3 Pages: 617-627

    • DOI

      10.1111/jre.12430

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Prolonged Survival of Transplanted Osteoblastic Cells Does Not Directly Accelerate the Healing of Calvarial Bone Defects.2016

    • Author(s)
      Kitami M, Kaku M, Rocabado JM, Ida T, Akiba N, Uoshima K.
    • Journal Title

      J Cell Physiol.

      Volume: 232 Issue: 9 Pages: 1974-82

    • DOI

      10.1002/jcp.25302

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mechanical Loading Stimulates Expression of Collagen Cross-Linking Associated Enzymes in Periodontal Ligament.2015

    • Author(s)
      Kaku M, Rosales Rocabado JM, Kitami M, Ida T, Akiba Y, Yamauchi M, Uoshima K.
    • Journal Title

      J Cell Physiol.

      Volume: 231(4) Issue: 4 Pages: 926-933

    • DOI

      10.1002/jcp.25184

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant

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Published: 2015-04-16   Modified: 2022-02-21  

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